C-reaction protein detection in human saliva by nanoplasmonic color imaging

Wenjun Hu, Tang Dang, Zhenyu Li, Linping Lei, Guoping Wang, Yuan Li, Hao Xu, Zongguang Zhou, Gang Logan Liu

Research output: Contribution to journalArticle

Abstract

A label-free and highly sensitive imaging sensor based on plasmonic-photonic interaction in a gold-titanium dioxidegold metal-insulator-metal (MIM) plasmonic nanocup array is reported. The sensor can detect proteins and exhibits superior performance in visible light sensing. This device enables very sensitive detection of an increase in superstrate refractive index based on changes in the red channel intensity of color imaging. Compared to other conventional plasmonic sensors, our device achieves transmission imaging detection by using normal white light and minimizes instrumentation requirement. In this study, we used the device to detect C-reaction protein (CRP) level in saliva, which is widely tested to help make clinical decisions in different diseases and disorders. The intensity imaging showed a good linear response between CRP concentration (from 5 to 100 ng/mL) and relative intensity change in the device. The lowest concentration of CRP that could be detected was 5 ng/mL. Moreover, it could achieve a positive detection in saliva from patients when the CRP level in serum was 3.2 μg/mL. Owing to the high performance of the MIM plasmonic nanocup array, the proposed device is promising for future portable optical sensing with visible light illumination and imaging.

Original languageEnglish (US)
Pages (from-to)1724-1733
Number of pages10
JournalJournal of Biomedical Nanotechnology
Volume16
Issue number8
DOIs
StatePublished - Jan 1 2019

Fingerprint

Protein C
Saliva
Color
Proteins
Imaging techniques
Equipment and Supplies
Metals
Light
Sensors
Optics and Photonics
Refractometry
Titanium
Lighting
Gold
Photonics
Labels
Refractive index
Serum

Keywords

  • Biosensor
  • Color imaging
  • Intensity imaging
  • Nanocavity
  • Surface plasmon resonance
  • Visible light

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

Cite this

C-reaction protein detection in human saliva by nanoplasmonic color imaging. / Hu, Wenjun; Dang, Tang; Li, Zhenyu; Lei, Linping; Wang, Guoping; Li, Yuan; Xu, Hao; Zhou, Zongguang; Liu, Gang Logan.

In: Journal of Biomedical Nanotechnology, Vol. 16, No. 8, 01.01.2019, p. 1724-1733.

Research output: Contribution to journalArticle

Hu, W, Dang, T, Li, Z, Lei, L, Wang, G, Li, Y, Xu, H, Zhou, Z & Liu, GL 2019, 'C-reaction protein detection in human saliva by nanoplasmonic color imaging', Journal of Biomedical Nanotechnology, vol. 16, no. 8, pp. 1724-1733. https://doi.org/10.1166/jbn.2019.2769
Hu, Wenjun ; Dang, Tang ; Li, Zhenyu ; Lei, Linping ; Wang, Guoping ; Li, Yuan ; Xu, Hao ; Zhou, Zongguang ; Liu, Gang Logan. / C-reaction protein detection in human saliva by nanoplasmonic color imaging. In: Journal of Biomedical Nanotechnology. 2019 ; Vol. 16, No. 8. pp. 1724-1733.
@article{ead138871e4f474898c312c2f699e0cf,
title = "C-reaction protein detection in human saliva by nanoplasmonic color imaging",
abstract = "A label-free and highly sensitive imaging sensor based on plasmonic-photonic interaction in a gold-titanium dioxidegold metal-insulator-metal (MIM) plasmonic nanocup array is reported. The sensor can detect proteins and exhibits superior performance in visible light sensing. This device enables very sensitive detection of an increase in superstrate refractive index based on changes in the red channel intensity of color imaging. Compared to other conventional plasmonic sensors, our device achieves transmission imaging detection by using normal white light and minimizes instrumentation requirement. In this study, we used the device to detect C-reaction protein (CRP) level in saliva, which is widely tested to help make clinical decisions in different diseases and disorders. The intensity imaging showed a good linear response between CRP concentration (from 5 to 100 ng/mL) and relative intensity change in the device. The lowest concentration of CRP that could be detected was 5 ng/mL. Moreover, it could achieve a positive detection in saliva from patients when the CRP level in serum was 3.2 μg/mL. Owing to the high performance of the MIM plasmonic nanocup array, the proposed device is promising for future portable optical sensing with visible light illumination and imaging.",
keywords = "Biosensor, Color imaging, Intensity imaging, Nanocavity, Surface plasmon resonance, Visible light",
author = "Wenjun Hu and Tang Dang and Zhenyu Li and Linping Lei and Guoping Wang and Yuan Li and Hao Xu and Zongguang Zhou and Liu, {Gang Logan}",
year = "2019",
month = "1",
day = "1",
doi = "10.1166/jbn.2019.2769",
language = "English (US)",
volume = "16",
pages = "1724--1733",
journal = "Journal of Biomedical Nanotechnology",
issn = "1550-7033",
publisher = "American Scientific Publishers",
number = "8",

}

TY - JOUR

T1 - C-reaction protein detection in human saliva by nanoplasmonic color imaging

AU - Hu, Wenjun

AU - Dang, Tang

AU - Li, Zhenyu

AU - Lei, Linping

AU - Wang, Guoping

AU - Li, Yuan

AU - Xu, Hao

AU - Zhou, Zongguang

AU - Liu, Gang Logan

PY - 2019/1/1

Y1 - 2019/1/1

N2 - A label-free and highly sensitive imaging sensor based on plasmonic-photonic interaction in a gold-titanium dioxidegold metal-insulator-metal (MIM) plasmonic nanocup array is reported. The sensor can detect proteins and exhibits superior performance in visible light sensing. This device enables very sensitive detection of an increase in superstrate refractive index based on changes in the red channel intensity of color imaging. Compared to other conventional plasmonic sensors, our device achieves transmission imaging detection by using normal white light and minimizes instrumentation requirement. In this study, we used the device to detect C-reaction protein (CRP) level in saliva, which is widely tested to help make clinical decisions in different diseases and disorders. The intensity imaging showed a good linear response between CRP concentration (from 5 to 100 ng/mL) and relative intensity change in the device. The lowest concentration of CRP that could be detected was 5 ng/mL. Moreover, it could achieve a positive detection in saliva from patients when the CRP level in serum was 3.2 μg/mL. Owing to the high performance of the MIM plasmonic nanocup array, the proposed device is promising for future portable optical sensing with visible light illumination and imaging.

AB - A label-free and highly sensitive imaging sensor based on plasmonic-photonic interaction in a gold-titanium dioxidegold metal-insulator-metal (MIM) plasmonic nanocup array is reported. The sensor can detect proteins and exhibits superior performance in visible light sensing. This device enables very sensitive detection of an increase in superstrate refractive index based on changes in the red channel intensity of color imaging. Compared to other conventional plasmonic sensors, our device achieves transmission imaging detection by using normal white light and minimizes instrumentation requirement. In this study, we used the device to detect C-reaction protein (CRP) level in saliva, which is widely tested to help make clinical decisions in different diseases and disorders. The intensity imaging showed a good linear response between CRP concentration (from 5 to 100 ng/mL) and relative intensity change in the device. The lowest concentration of CRP that could be detected was 5 ng/mL. Moreover, it could achieve a positive detection in saliva from patients when the CRP level in serum was 3.2 μg/mL. Owing to the high performance of the MIM plasmonic nanocup array, the proposed device is promising for future portable optical sensing with visible light illumination and imaging.

KW - Biosensor

KW - Color imaging

KW - Intensity imaging

KW - Nanocavity

KW - Surface plasmon resonance

KW - Visible light

UR - http://www.scopus.com/inward/record.url?scp=85068492722&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85068492722&partnerID=8YFLogxK

U2 - 10.1166/jbn.2019.2769

DO - 10.1166/jbn.2019.2769

M3 - Article

C2 - 31219011

AN - SCOPUS:85068492722

VL - 16

SP - 1724

EP - 1733

JO - Journal of Biomedical Nanotechnology

JF - Journal of Biomedical Nanotechnology

SN - 1550-7033

IS - 8

ER -