@article{9e36043064344e27ab10a54bcdccafea,
title = "Brucella ceti sequence type 23, 26, and 27 infections in North American cetaceans",
abstract = "Brucella ceti infection is associated with a variety of disease outcomes in cetaceans globally. Multiple genotypes of B. ceti have been identified. This retrospective aimed to determine if specific lesions were associated with different B. ceti DNA sequence types (STs). Characterization of ST was performed on 163 samples from 88 free-ranging cetaceans, including common bottlenose dolphin Tursiops truncatus (T.t.; n = 73), common short-beaked dolphin Delphinus delphis (D.d.; n = 7), striped dolphin Stenella coeruleoalba (n = 3), Pacific white-sided dolphin Lageno - rhynchus obliquidens (n = 2), sperm whale Physeter macrocephalus (n = 2), and harbour porpoise Phocoena phocoena (n = 1), that stranded along the coast of the US mainland and Hawaii. ST was determined using a previously described insertion sequence 711 quantitative PCR. Concordance with 9-locus multi-locus sequence typing was assessed in a subset of samples (n = 18). ST 26 was most commonly identified in adult dolphins along the US east coast with non-suppurative meningoencephalitis (p = 0.009). Animals infected with ST 27 were predominately perinates that were aborted or died shortly after birth with evidence of in utero pneumonia (p = 0.035). Reproductive tract inflammation and meningoencephalitis were also observed in adult T.t. and D.d. with ST 27, though low sample size limited interpretation. ST 23 infections can cause disease in cetacean families other than porpoises (Phocoenidae), including neurobrucellosis in D.d. In total, 11 animals were potentially infected with multiple STs. These data indicate differences in pathogenesis among B. ceti STs in free-ranging cetaceans, and infection with multiple STs is possible.",
keywords = "Bottlenose dolphin, Brucella ceti, Brucellosis, Cetacean, Delphinus delphis, Dolphins, IS711, Pathology, Tursiops truncatus",
author = "Curtiss, {Jeffrey B.} and Colegrove, {Kathleen M.} and Allison Dianis and Kinsel, {Michael J.} and Nadia Ahmed and Deborah Fauquier and Teresa Rowles and Misty Niemeyer and Rotstein, {David S.} and Maddox, {Carol W.} and Terio, {Karen A.}",
note = "Funding Information: Acknowledgements. This work could not have been conducted without the efforts of the many US MMSN members, including additional personnel from those agencies working on the northern GoM unusual mortality event (UME) and the Mid-Atlantic UME. The authors acknowledge the UME Investigative Team members and the past and present members of the Working Group for Marine Mammal Un - usual Mortality Events. We thank Blair Mase-Guthrie and Elizabeth Stratton for coordination of the NMFS Southeast Region, Mendy Garron for coordination of the NMFS Greater Atlantic Region Stranding Network, Kristin Wilkinson and Justin Viezbicke for coordination of the NMFS West Coast Stranding Network. We thank those institutions which contributed samples to this project including the Institute For Marine Mammal Studies, International Fund for Animal Welfare, Oregon State University, University of North Carolina at Wilmington, MMSN for the Central Coast of North Carolina, NOAA National Ocean Service Center for Coastal Environmental Health and Biomolecular Research, Hawai{\textquoteright}i Pacific University, Virginia Aquarium and Marine Science Center, Georgia Department of Natural Resources, Dauphin Island Sea Lab, Louisiana Department of Fish and Game, The Marine Mammal Center, Riverhead Foundation for Marine Research and Preservation, Atlantic Marine Conservation Society, New Jersey Department of Agriculture, Santa Barbara Museum of Natural History, and Mote Marine Laboratory. We also thank Daniel Woodburn for laboratory assistance and Anne Kimmerlein for assistance in statistical analysis. Tracey Goldstein and Jerry Saliki from the University of California Davis and the University of Georgia, respectively are acknowledged for morbillivirus molecular diagnostics. A.D. was supported by funding from the University of Illinois College of Veterinary Medicine through the Summer Research Training Program. Funding for necropsy work and sample analysis was provided in part by the NOAA John H. Prescott Stranding Grant Program, Grant #: NA11NMF4390079. All responses, necropsies, and sample collection were conducted under Stranding Agreements between the individual network members and NOAA{\textquoteright}s National Marine Fisheries Service. The scientific results and conclusions, as well as any views or opinions expressed herein, are those of the authors and do not necessarily reflect the views of NOAA. Publisher Copyright: {\textcopyright} 2022 Inter-Research. All rights reserved.",
year = "2022",
doi = "10.3354/dao03644",
language = "English (US)",
volume = "148",
pages = "57--72",
journal = "Diseases of Aquatic Organisms",
issn = "0177-5103",
publisher = "Inter-Research",
}