Broccoli consumption affects the human gastrointestinal microbiota

Jennifer L. Kaczmarek, Xiaoji Liu, Craig S. Charron, Janet A. Novotny, Elizabeth H Jeffery, Harold E. Seifried, Sharon A. Ross, Michael J Miller, Kelly S Swanson, Hannah Diane Holscher

Research output: Contribution to journalArticle

Abstract

The human gastrointestinal microbiota is increasingly linked to health outcomes; however, our understanding of how specific foods alter the microbiota is limited. Cruciferous vegetables such as broccoli are a good source of dietary fiber and phytonutrients, including glucosinolates, which can be metabolized by gastrointestinal microbes. This study aimed to determine the impact of broccoli consumption on the gastrointestinal microbiota of healthy adults. A controlled feeding, randomized, crossover study consisting of two 18-day treatment periods separated by a 24-day washout was conducted in healthy adults (n=18). Participants were fed at weight maintenance with the intervention period diet including 200 g of cooked broccoli and 20 g of raw daikon radish per day. Fecal samples were collected at baseline and at the end of each treatment period for microbial analysis. Beta diversity analysis indicated that bacterial communities were impacted by treatment (P=.03). Broccoli consumption decreased the relative abundance of Firmicutes by 9% compared to control (P=.05), increased the relative abundance of Bacteroidetes by 10% compared to control (P=.03) and increased Bacteroides by 8% relative to control (P=.02). Furthermore, the effects were strongest among participants with body mass index <26 kg/m2, and within this group, there were associations between bacterial relative abundance and glucosinolate metabolites. Functional prediction revealed that broccoli consumption increased the pathways involved in the functions of the endocrine system (P=.05), transport and catabolism (P=.04), and energy metabolism (P=.01). These results reveal that broccoli consumption affects the composition and function of the human gastrointestinal microbiota.

LanguageEnglish (US)
Pages27-34
Number of pages8
JournalJournal of Nutritional Biochemistry
Volume63
DOIs
StatePublished - Jan 1 2019

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Brassica
Glucosinolates
Vegetables
Phytochemicals
Nutrition
Metabolites
Health
Bacteroidetes
Raphanus
Bacteroides
Endocrine System
Microbiota
Dietary Fiber
Chemical analysis
Cross-Over Studies
Energy Metabolism
Gastrointestinal Microbiome
Body Mass Index
Therapeutics
Maintenance

Keywords

  • Bacteroides
  • Brassica vegetables
  • Cruciferous vegetables
  • Glucosinolates
  • Microbiome

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology
  • Nutrition and Dietetics
  • Clinical Biochemistry

Cite this

Broccoli consumption affects the human gastrointestinal microbiota. / Kaczmarek, Jennifer L.; Liu, Xiaoji; Charron, Craig S.; Novotny, Janet A.; Jeffery, Elizabeth H; Seifried, Harold E.; Ross, Sharon A.; Miller, Michael J; Swanson, Kelly S; Holscher, Hannah Diane.

In: Journal of Nutritional Biochemistry, Vol. 63, 01.01.2019, p. 27-34.

Research output: Contribution to journalArticle

Kaczmarek, Jennifer L. ; Liu, Xiaoji ; Charron, Craig S. ; Novotny, Janet A. ; Jeffery, Elizabeth H ; Seifried, Harold E. ; Ross, Sharon A. ; Miller, Michael J ; Swanson, Kelly S ; Holscher, Hannah Diane. / Broccoli consumption affects the human gastrointestinal microbiota. In: Journal of Nutritional Biochemistry. 2019 ; Vol. 63. pp. 27-34.
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