Abstract
Optical microscopy is a powerful tool for understanding the fundamentals of the microscopic world. However, for centuries its resolving ability remained limited by the optical diffraction limit. Super-resolution fluorescence microscopy (SRFM) has been introduced to break the diffraction limit and significantly expand the fields in which optical microscopy can be applied. Unfortunately, SRFM contributes little towards axial resolution enhancement, rendering observation of the axial and three-dimensional structures of biological tissues difficult; this may yield a misunderstanding of intracellular interactions. Based on the existing literature, the development of axial SRFM is still behind that of lateral SRFM. In light of this, this Review presents a comprehensive summary of the principles, development, characteristics, and applications of existing techniques for improving the axial resolution. This Review will provide a guide to researchers and promote further development of related technology.
| Original language | English (US) |
|---|---|
| Article number | 1700333 |
| Journal | Laser and Photonics Reviews |
| Volume | 12 |
| Issue number | 8 |
| DOIs | |
| State | Published - Aug 2018 |
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Keywords
- axial localization precision
- axial resolution
- axial single particle localization
- axial single particle tracking
- axial super-resolution fluorescence microscopy
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Condensed Matter Physics
Cite this
Breaking the Axial Diffraction Limit : A Guide to Axial Super-Resolution Fluorescence Microscopy. / Liu, Wenjie; Toussaint, Kimani C.; Okoro, Chukwuemeka; Zhu, Dazhao; Chen, Youhua; Kuang, Cuifang; Liu, Xu.
In: Laser and Photonics Reviews, Vol. 12, No. 8, 1700333, 08.2018.Research output: Contribution to journal › Review article
}
TY - JOUR
T1 - Breaking the Axial Diffraction Limit
T2 - A Guide to Axial Super-Resolution Fluorescence Microscopy
AU - Liu, Wenjie
AU - Toussaint, Kimani C.
AU - Okoro, Chukwuemeka
AU - Zhu, Dazhao
AU - Chen, Youhua
AU - Kuang, Cuifang
AU - Liu, Xu
PY - 2018/8
Y1 - 2018/8
N2 - Optical microscopy is a powerful tool for understanding the fundamentals of the microscopic world. However, for centuries its resolving ability remained limited by the optical diffraction limit. Super-resolution fluorescence microscopy (SRFM) has been introduced to break the diffraction limit and significantly expand the fields in which optical microscopy can be applied. Unfortunately, SRFM contributes little towards axial resolution enhancement, rendering observation of the axial and three-dimensional structures of biological tissues difficult; this may yield a misunderstanding of intracellular interactions. Based on the existing literature, the development of axial SRFM is still behind that of lateral SRFM. In light of this, this Review presents a comprehensive summary of the principles, development, characteristics, and applications of existing techniques for improving the axial resolution. This Review will provide a guide to researchers and promote further development of related technology.
AB - Optical microscopy is a powerful tool for understanding the fundamentals of the microscopic world. However, for centuries its resolving ability remained limited by the optical diffraction limit. Super-resolution fluorescence microscopy (SRFM) has been introduced to break the diffraction limit and significantly expand the fields in which optical microscopy can be applied. Unfortunately, SRFM contributes little towards axial resolution enhancement, rendering observation of the axial and three-dimensional structures of biological tissues difficult; this may yield a misunderstanding of intracellular interactions. Based on the existing literature, the development of axial SRFM is still behind that of lateral SRFM. In light of this, this Review presents a comprehensive summary of the principles, development, characteristics, and applications of existing techniques for improving the axial resolution. This Review will provide a guide to researchers and promote further development of related technology.
KW - axial localization precision
KW - axial resolution
KW - axial single particle localization
KW - axial single particle tracking
KW - axial super-resolution fluorescence microscopy
UR - http://www.scopus.com/inward/record.url?scp=85047807355&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85047807355&partnerID=8YFLogxK
U2 - 10.1002/lpor.201700333
DO - 10.1002/lpor.201700333
M3 - Review article
AN - SCOPUS:85047807355
VL - 12
JO - Laser and Photonics Reviews
JF - Laser and Photonics Reviews
SN - 1863-8880
IS - 8
M1 - 1700333
ER -