TY - JOUR
T1 - Breaking the Axial Diffraction Limit
T2 - A Guide to Axial Super-Resolution Fluorescence Microscopy
AU - Liu, Wenjie
AU - Toussaint, Kimani C.
AU - Okoro, Chukwuemeka
AU - Zhu, Dazhao
AU - Chen, Youhua
AU - Kuang, Cuifang
AU - Liu, Xu
N1 - Funding Information:
This work was sponsored by National Basic Research Program of China (973 Program) (2015CB352003); National Key Research and Development Program of China (2017YFC0110303, 2016YFF0101400); National Natural Science Foundation of China (NSFC) (61335003, 61427818); Natural Science Foundation of Zhejiang province (LR16F050001); and the Fundamental Research Funds for the Central Universities (2017FZA5004).
Publisher Copyright:
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2018/8
Y1 - 2018/8
N2 - Optical microscopy is a powerful tool for understanding the fundamentals of the microscopic world. However, for centuries its resolving ability remained limited by the optical diffraction limit. Super-resolution fluorescence microscopy (SRFM) has been introduced to break the diffraction limit and significantly expand the fields in which optical microscopy can be applied. Unfortunately, SRFM contributes little towards axial resolution enhancement, rendering observation of the axial and three-dimensional structures of biological tissues difficult; this may yield a misunderstanding of intracellular interactions. Based on the existing literature, the development of axial SRFM is still behind that of lateral SRFM. In light of this, this Review presents a comprehensive summary of the principles, development, characteristics, and applications of existing techniques for improving the axial resolution. This Review will provide a guide to researchers and promote further development of related technology.
AB - Optical microscopy is a powerful tool for understanding the fundamentals of the microscopic world. However, for centuries its resolving ability remained limited by the optical diffraction limit. Super-resolution fluorescence microscopy (SRFM) has been introduced to break the diffraction limit and significantly expand the fields in which optical microscopy can be applied. Unfortunately, SRFM contributes little towards axial resolution enhancement, rendering observation of the axial and three-dimensional structures of biological tissues difficult; this may yield a misunderstanding of intracellular interactions. Based on the existing literature, the development of axial SRFM is still behind that of lateral SRFM. In light of this, this Review presents a comprehensive summary of the principles, development, characteristics, and applications of existing techniques for improving the axial resolution. This Review will provide a guide to researchers and promote further development of related technology.
KW - axial localization precision
KW - axial resolution
KW - axial single particle localization
KW - axial single particle tracking
KW - axial super-resolution fluorescence microscopy
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U2 - 10.1002/lpor.201700333
DO - 10.1002/lpor.201700333
M3 - Review article
AN - SCOPUS:85047807355
VL - 12
JO - Laser and Photonics Reviews
JF - Laser and Photonics Reviews
SN - 1863-8880
IS - 8
M1 - 1700333
ER -