Brassinosteroid and gibberellin control of seedling traits in maize (Zea mays L.)

Songlin Hu, Darlene L. Sanchez, Cuiling Wang, Alexander E. Lipka, Yanhai Yin, Candice A.C. Gardner, Thomas Lübberstedt

Research output: Contribution to journalArticle

Abstract

In this study, we established two doubled haploid (DH) libraries with a total of 207 DH lines. We applied BR and GA inhibitors to all DH lines at seedling stage and measured seedling BR and GA inhibitor responses. Moreover, we evaluated field traits for each DH line (untreated). We conducted genome-wide association studies (GWAS) with 62,049 genome wide SNPs to explore the genetic control of seedling traits by BR and GA. In addition, we correlate seedling stage hormone inhibitor response with field traits. Large variation for BR and GA inhibitor response and field traits was observed across these DH lines. Seedling stage BR and GA inhibitor response was significantly correlate with yield and flowering time. Using three different GWAS approaches to balance false positive/negatives, multiple SNPs were discovered to be significantly associated with BR/GA inhibitor responses with some localized within gene models. SNPs from gene model GRMZM2G013391 were associated with GA inhibitor response across all three GWAS models. This gene is expressed in roots and shoots and was shown to regulate GA signaling. These results show that BRs and GAs have a great impact for controlling seedling growth. Gene models from GWAS results could be targets for seeding traits improvement.

Original languageEnglish (US)
Pages (from-to)132-141
Number of pages10
JournalPlant Science
Volume263
DOIs
StatePublished - Oct 2017

Keywords

  • Brassinosteroid
  • Field traits
  • Gibberellins
  • Seedling traits
  • Zea mays

ASJC Scopus subject areas

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

Fingerprint Dive into the research topics of 'Brassinosteroid and gibberellin control of seedling traits in maize (Zea mays L.)'. Together they form a unique fingerprint.

  • Cite this

    Hu, S., Sanchez, D. L., Wang, C., Lipka, A. E., Yin, Y., Gardner, C. A. C., & Lübberstedt, T. (2017). Brassinosteroid and gibberellin control of seedling traits in maize (Zea mays L.). Plant Science, 263, 132-141. https://doi.org/10.1016/j.plantsci.2017.07.011