Blood screening using diffraction phase cytometry

Mustafa Mir, Huafeng Ding, Zhuo Wang, Jason Reedy, Krishnarao Tangella, Gabriel Popescu

Research output: Contribution to journalArticlepeer-review

Abstract

Blood smear analysis has remained a crucial diagnostic tool for pathologists despite the advent of automatic analyzers such as flow cytometers and impedance counters. Though these current methods have proven to be indispensible tools for physicians and researchers alike, they provide limited information on the detailed morphology of individual cells, and merely alert the operator to manually examine a blood smear by raising flags when abnormalities are detected. We demonstrate an automatic interferometry-based smear analysis technique known as diffraction phase cytometry (DPC), which is capable of providing the same information on red blood cells as is provided by current clinical analyzers, while rendering additional, currently unavailable parameters on the 2-D and 3-D morphology of individual red blood cells. To validate the utility of our technique in a clinical setting, we present a comparison between tests generated from 32 patients by a state of the art clinical impedance counter and DPC.

Original languageEnglish (US)
Article number027016
JournalJournal of biomedical optics
Volume15
Issue number2
DOIs
StatePublished - 2010

Keywords

  • Blood smear
  • Diffraction phase cytometry
  • Mean cell hemoglobin concentration
  • Mean corpuscular volume
  • Quantitative phase imaging
  • Red blood cell
  • Refractive index
  • Single cell analysis
  • Sphericity

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

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