Blockade of cathepsin B expression in human glioblastoma cells is associated with suppression of angiogenesis

Niranjan Yanamandra, Krishna V. Gumidyala, Kevin G. Waldron, Meena Gujrati, William C. Olivero, Dzung H. Dinh, Jasti S. Rao, Sanjeeva Mohanam

Research output: Contribution to journalArticle

Abstract

The cysteine proteinase cathepsin B has been implicated in tumor progression by virtue of its increased mRNA and protein levels, as well as its localization at the invading front of the tumor. In this study, we examined whether blocking cathepsin B expression in human glioblastoma SNB19 cells affects angiogenesis. Stable transfectants of human glioblastoma cells with a plasmid containing antisense cathepsin B cDNA showed decreased migration rates in wound- and spheroid-migration assays. Analysis showed a reduction in VEGF protein and MMP-9 activity in the cathepsin B antisense cDNA-transfected cells. Regarding angiogenesis in vitro, we found that the conditioned medium of glioblastoma cells with downregulated cathepsin B expression reduced cell-cell interaction of human microvascular endothelial cells, resulting in the disruption of capillary-like network formation. Furthermore, a marked reduction in microvasculature development was seen in an in vivo dorsal air sac assay of glioblastoma cells with downregulated cathepsin B expression. Taken together, these results provide evidence that inhibition of cathepsin B expression can suppress glioblastoma-induced neovascularization.

Original languageEnglish (US)
Pages (from-to)2224-2230
Number of pages7
JournalOncogene
Volume23
Issue number12
DOIs
StatePublished - Mar 18 2004

Fingerprint

Cathepsin B
Glioblastoma
yeast proteinase B
Down-Regulation
Complementary DNA
Air Sacs
Cysteine Proteases
Conditioned Culture Medium
Microvessels
Matrix Metalloproteinases
Cell Communication
Vascular Endothelial Growth Factor A
Neoplasms
Proteins
Plasmids
Endothelial Cells
Messenger RNA
Wounds and Injuries

Keywords

  • Angiogenesis
  • Antisense
  • Cathepsin B
  • Glioblastoma

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Yanamandra, N., Gumidyala, K. V., Waldron, K. G., Gujrati, M., Olivero, W. C., Dinh, D. H., ... Mohanam, S. (2004). Blockade of cathepsin B expression in human glioblastoma cells is associated with suppression of angiogenesis. Oncogene, 23(12), 2224-2230. https://doi.org/10.1038/sj.onc.1207338

Blockade of cathepsin B expression in human glioblastoma cells is associated with suppression of angiogenesis. / Yanamandra, Niranjan; Gumidyala, Krishna V.; Waldron, Kevin G.; Gujrati, Meena; Olivero, William C.; Dinh, Dzung H.; Rao, Jasti S.; Mohanam, Sanjeeva.

In: Oncogene, Vol. 23, No. 12, 18.03.2004, p. 2224-2230.

Research output: Contribution to journalArticle

Yanamandra, N, Gumidyala, KV, Waldron, KG, Gujrati, M, Olivero, WC, Dinh, DH, Rao, JS & Mohanam, S 2004, 'Blockade of cathepsin B expression in human glioblastoma cells is associated with suppression of angiogenesis', Oncogene, vol. 23, no. 12, pp. 2224-2230. https://doi.org/10.1038/sj.onc.1207338
Yanamandra N, Gumidyala KV, Waldron KG, Gujrati M, Olivero WC, Dinh DH et al. Blockade of cathepsin B expression in human glioblastoma cells is associated with suppression of angiogenesis. Oncogene. 2004 Mar 18;23(12):2224-2230. https://doi.org/10.1038/sj.onc.1207338
Yanamandra, Niranjan ; Gumidyala, Krishna V. ; Waldron, Kevin G. ; Gujrati, Meena ; Olivero, William C. ; Dinh, Dzung H. ; Rao, Jasti S. ; Mohanam, Sanjeeva. / Blockade of cathepsin B expression in human glioblastoma cells is associated with suppression of angiogenesis. In: Oncogene. 2004 ; Vol. 23, No. 12. pp. 2224-2230.
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