Bisphosphonate inhibition of phosphoglycerate kinase: Quantitative structure-activity relationship and pharmacophore modeling investigation

Evangelia Kotsikorou, Gurmukh Sahota, Eric Oldfield

Research output: Contribution to journalArticle

Abstract

We report the results of a three-dimensional quantitative structure-activity relationship (3D-QSAR) and pharmacophore modeling investigation of the interaction of the enzyme 3-phosphoglycerate kinase (PGK) with aryl and alkyl bisphosphonates. For the human enzyme, the IC50 values are predicted within a factor of 2 over the 240× experimental range in activity, while for the yeast enzyme, binding of the more flexible alkyl bisphosphonates is predicted within a factor of ∼4 (over a 2500× range in activity). Pharmacophore models indicate the importance of two negative ionizable features, one hydrophobic feature, and one halogen feature, and docking studies indicate that bisphosphonates bind in a manner similar to the 3-phosphoglycerate molecule identified crystallographically, The results give a good account of the activities of a diverse range of bisphosphonate inhibitors and are of interest in the context of developing inhibitors of glycolysis in organisms that are totally reliant on glycolysis for ATP production, such as trypanosomatid parasites.

Original languageEnglish (US)
Pages (from-to)6692-6703
Number of pages12
JournalJournal of Medicinal Chemistry
Volume49
Issue number23
DOIs
StatePublished - Nov 16 2006

ASJC Scopus subject areas

  • Molecular Medicine
  • Drug Discovery

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