TY - JOUR
T1 - Bipolar localization of the replication origin regions of chromosomes in vegetative and sporulating cells of B. subtilis
AU - Webb, Chris D.
AU - Teleman, Aurelio
AU - Gordon, Scott
AU - Straight, Aaron
AU - Belmont, Andrew
AU - Lin, Daniel Chi Hong
AU - Grossman, Alan D.
AU - Wright, Andrew
AU - Losick, Richard
N1 - Funding Information:
We thank S. Meyer for plasmid pSM79, D. Garsin for plasmid pDAG8-1, P. Stragier for plasmids pDG795 and pDG1832, and O. Resnekov for invaluable discussions and help with immunofluorescence experiments. C. D. W. is a predoctoral fellow of the National Science Foundation. This work was supported by NIH grants GM38035 to A. W. and GM18568 to R. L.
PY - 1997/3/7
Y1 - 1997/3/7
N2 - To investigate chromosome segregation in B. subtilis, we introduced tandem copies of the lactose operon operator into the chromosome near the replication origin or terminus. We then visualized the position of the operator cassettes with green fluorescent protein fused to the LacI repressor. In sporulating bacteria, which undergo asymmetric cell division, origins localized near each pole of the cell whereas termini were restricted to the middle. In growing cells, which undergo binary fission, origins were observed at various positions but preferentially toward the poles early in the cell cycle. In contrast, termini showed little preference for the poles. These results indicate the existence of a mitotic-like apparatus that is responsible for moving the origin regions of newly formed chromosomes toward opposite ends of the cell.
AB - To investigate chromosome segregation in B. subtilis, we introduced tandem copies of the lactose operon operator into the chromosome near the replication origin or terminus. We then visualized the position of the operator cassettes with green fluorescent protein fused to the LacI repressor. In sporulating bacteria, which undergo asymmetric cell division, origins localized near each pole of the cell whereas termini were restricted to the middle. In growing cells, which undergo binary fission, origins were observed at various positions but preferentially toward the poles early in the cell cycle. In contrast, termini showed little preference for the poles. These results indicate the existence of a mitotic-like apparatus that is responsible for moving the origin regions of newly formed chromosomes toward opposite ends of the cell.
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U2 - 10.1016/S0092-8674(00)81909-1
DO - 10.1016/S0092-8674(00)81909-1
M3 - Article
C2 - 9054506
AN - SCOPUS:0030901736
VL - 88
SP - 667
EP - 674
JO - Cell
JF - Cell
SN - 0092-8674
IS - 5
ER -