Bipolar localization of the replication origin regions of chromosomes in vegetative and sporulating cells of B. subtilis

Chris D. Webb, Aurelio Teleman, Scott Gordon, Aaron Straight, Andrew Belmont, Daniel Chi Hong Lin, Alan D. Grossman, Andrew Wright, Richard Losick

Research output: Contribution to journalArticlepeer-review

Abstract

To investigate chromosome segregation in B. subtilis, we introduced tandem copies of the lactose operon operator into the chromosome near the replication origin or terminus. We then visualized the position of the operator cassettes with green fluorescent protein fused to the LacI repressor. In sporulating bacteria, which undergo asymmetric cell division, origins localized near each pole of the cell whereas termini were restricted to the middle. In growing cells, which undergo binary fission, origins were observed at various positions but preferentially toward the poles early in the cell cycle. In contrast, termini showed little preference for the poles. These results indicate the existence of a mitotic-like apparatus that is responsible for moving the origin regions of newly formed chromosomes toward opposite ends of the cell.

Original languageEnglish (US)
Pages (from-to)667-674
Number of pages8
JournalCell
Volume88
Issue number5
DOIs
StatePublished - Mar 7 1997

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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