Biotype and ribotype diversity among lactobacillus isolates from the mouse ileum

Research output: Contribution to journalArticle

Abstract

The present study evaluated the relative diversity of lactobacilli in the mouse ileum as defined by conventional phenotypic characteristics versus ribotype patterns. Forty-one Lactobacillus isolates were first presumptively identified using data from carbohydrate fermentation and biochemical tests. Numerical analysis of the biotype data placed a total of four Lactobacillus species into three major clusters and six small clusters. Most of the isolates were typed as L. gasseri or L. fermentum. To further distinguish these isolates, restriction fragment length polymorphism (RFLP) analysis was performed using a 23S rRNA gene probe to develop ribotypes and allow clustering to genotypic groups. Numerical analysis of the ribotype data also revealed three main clusters, with identical grouping to those generated by the biotype data, with only one exception. Thus, for both the ribotype and biotype data the isolates were generally conserved within clusters. A direct microscopic-Gram-stain procedure was used to determine the relative adhesion capacity of the mouse ileum isolates. The Lactobacillus isolates varied widely in their ability to colonize a colonic epithelial cell line with no apparent relationship between adhesion phenotype and ribotype or biotype characteristics.

Original languageEnglish (US)
Pages (from-to)423-431
Number of pages9
JournalSystematic and Applied Microbiology
Volume20
Issue number3
DOIs
StatePublished - Aug 1997

Fingerprint

Ribotyping
ribotypes
biotype
Lactobacillus
biotypes
Ileum
ileum
mice
adhesion
Lactobacillus gasseri
Lactobacillus fermentum
rRNA Genes
Restriction Fragment Length Polymorphisms
probes (equipment)
Fermentation
fermentation
Cluster Analysis
phenotype
carbohydrate
restriction fragment length polymorphism

Keywords

  • Lactobacillus
  • Mouse small intestine
  • Ribotyping
  • Taxonomy

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Applied Microbiology and Biotechnology

Cite this

Biotype and ribotype diversity among lactobacillus isolates from the mouse ileum. / Ning, Wei; Mackie, Roderick Ian; Gaskins, H Rex.

In: Systematic and Applied Microbiology, Vol. 20, No. 3, 08.1997, p. 423-431.

Research output: Contribution to journalArticle

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