Biosynthetic radiolabeling of bacterial lipopolysaccharide to high specific activity

Robert S. Munford, Linda C. DeVeaux, J. E. Cronan, Paul D. Rick

Research output: Contribution to journalArticlepeer-review


We describe a method for producing radiolabeled lipopolysaccharide (LPS) by incorporating [3H]acetate into an aceEF, gltA strain of Escherichia coli K12. The LPS has substantially greater specific radioactivity (2 μCi per μg LPS, or approximately 8 Ci/mmol) than has been reported previously for biosynthetically radiolabeled LPS. The 3H is incorporated into the fatty acyl chains of the lipid A moiety. LPS prepared by this method has several attractive features for biological studies, including native structure and bioactivity, long radioactive half-life, and high specific activity.

Original languageEnglish (US)
Pages (from-to)115-120
Number of pages6
JournalJournal of Immunological Methods
Issue number1-2
StatePublished - 1992


  • Endotoxin
  • Lipid A
  • Lipopolysaccharide
  • Radiolabel

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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