Abstract
We describe a method for producing radiolabeled lipopolysaccharide (LPS) by incorporating [3H]acetate into an aceEF, gltA strain of Escherichia coli K12. The LPS has substantially greater specific radioactivity (2 μCi per μg LPS, or approximately 8 Ci/mmol) than has been reported previously for biosynthetically radiolabeled LPS. The 3H is incorporated into the fatty acyl chains of the lipid A moiety. LPS prepared by this method has several attractive features for biological studies, including native structure and bioactivity, long radioactive half-life, and high specific activity.
Original language | English (US) |
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Pages (from-to) | 115-120 |
Number of pages | 6 |
Journal | Journal of Immunological Methods |
Volume | 148 |
Issue number | 1-2 |
DOIs | |
State | Published - 1992 |
Keywords
- Endotoxin
- Lipid A
- Lipopolysaccharide
- Radiolabel
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology