Biosynthesis and Characterization of 14C-Enriched Flavonoid Fractions from Plant Cell Suspension Cultures

Gad G. Yousef, David S. Seigler, Michael A. Grusak, Randy B. Rogers, Christopher T.G. Knight, Tristan F.B. Kraft, John W. Erdman, Mary Ann Lila

Research output: Contribution to journalArticlepeer-review

Abstract

A range of radiolabeled anthocyanins, proanthocyanidins, and other flavonoids were accumulated by cell suspension cultures of two plant species, ohelo (Vaccinium pahalae) and grape (a Vitis hybrid, Bailey Alicant A), after providing uniformly labeled [14C]sucrose to the medium. Approximately 15% of administered label was recovered in a series of flavonoid-rich fractions varying in composition. Anthocyanins, and monomers to oligomers of proanthocyanidins, were labeled effectively and characterized from both species. Most of the proanthocyanidin oligomers were based on the flavan-3-ols (+)-catechin and (-)-epicatechin. Cyanidin and peonidin glycosides were the dominant forms of anthocyanins in both species. Whereas the predominant form of flavonoids identified from ohelo cell cultures was proanthocyanidins, grape cell cultures produced mostly anthocyanins. The labeled phytochemicals were produced for use in subsequent in vivo animal feeding studies to gauge their bioavailability and accumulation in target organs.

Original languageEnglish (US)
Pages (from-to)1138-1145
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume52
Issue number5
DOIs
StatePublished - Mar 10 2004

Keywords

  • Anthocyanins
  • Flavonoid-rich fractions
  • Grape (Vitis)
  • Ohelo (Vaccinium)
  • Proanthocyanidins
  • Radiolabeling

ASJC Scopus subject areas

  • General Chemistry
  • General Agricultural and Biological Sciences

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