Biomimetic Virulomics for Capture and Identification of Cell-Type Specific Effector Proteins

John D. Lapek, Ronnie H. Fang, Xiaoli Wei, Pengyang Li, Bo Wang, Liangfang Zhang, David J. Gonzalez

Research output: Contribution to journalArticlepeer-review

Abstract

An unmet challenge in the study of disease is to accurately streamline the identification of important virulence factors. Traditional, genetically driven approaches miss biologically relevant markers due to discordance between the genome and proteome. Here, we developed a nanotechnology-enabled affinity enrichment strategy coupled with multiplexed quantitative proteomics, namely Biomimetic Virulomics, for successful identification of cell-type specific effector proteins of both prokaryotic and eukaryotic pathogens. We highlight the power of Biomimetic Virulomics by capturing known virulence factors in a high-throughput, cell-type guided fashion. Additionally, a comprehensive characterization of the membrane protein component of biomimetics utilized in this strategy is provided. Interfacing cell-derived nanomaterials with multiplexed quantitative proteomics allow for a specific targeting strategy of virulence factors that can be utilized for drug discovery against prominent human diseases.

Original languageEnglish (US)
Pages (from-to)11831-11838
Number of pages8
JournalACS Nano
Volume11
Issue number12
DOIs
StatePublished - Dec 26 2017
Externally publishedYes

Keywords

  • affinity enrichment
  • biomimetics
  • mass spectrometry
  • quantitative proteomics
  • virulence factors

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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