TY - JOUR
T1 - Biomimetic Approach to Promote Cellular Uptake and Enhance Photoacoustic Properties of Tumor-Seeking Dyes
AU - East, Amanda K.
AU - Lee, Michael C.
AU - Jiang, Chang
AU - Sikander, Qasim
AU - Chan, Jefferson
N1 - A.K.E. acknowledges the Beckman Institute Graduate Fellowship for previous financial support. J.C. thanks the Camille and Henry Dreyfus Foundation for support. Major funding for the 500 MHz Bruker CryoProbe was provided by the Roy J. Carver Charitable Trust (Muscatine, Iowa; Grant NO. 15-4521) to the School of Chemical Sciences NMR Lab. The Q-Tof Ultima mass spectrometer was purchased in part with a grant from the National Science Foundation, Division of Biological Infrastructure (DBI-0100085). The authors thank Prof. Stephen A. Boppart (Department Bioengineering, UIUC) for donation of the Maestro In-Vivo Fluorescence Imaging System. The authors would also like to thank Rodrigo Tapia Hernandez for performing DLS measurements, and Prof. Cathy Murphy for the use of the Malvern Panalytical Zetasizer.
A.K.E. acknowledges the Beckman Institute Graduate Fellowship for previous financial support. J.C. thanks the Camille and Henry Dreyfus Foundation for support. Major funding for the 500 MHz Bruker CryoProbe was provided by the Roy J. Carver Charitable Trust (Muscatine, Iowa; Grant NO. 15-4521) to the School of Chemical Sciences NMR Lab. The Q-Tof Ultima mass spectrometer was purchased in part with a grant from the National Science Foundation, Division of Biological Infrastructure (DBI-0100085). The authors thank Prof. Stephen A. Boppart (Department Bioengineering, UIUC) for donation of the Maestro In-Vivo Fluorescence Imaging System. The authors would also like to thank Rodrigo Tapia Hernandez for performing DLS measurements, and Prof. Cathy Murphy for the use of the Malvern Panalytical Zetasizer. This work was supported by the National Institutes of Health (R35GM133581).
This work was supported by the National Institutes of Health (R35GM133581).
PY - 2023/4/5
Y1 - 2023/4/5
N2 - The attachment of glucose to drugs and imaging agents enables cancer cell targeting via interactions with GLUT1 overexpressed on the cell surface. While an added benefit of this modification is the solubilizing effect of carbohydrates, in the context of imaging agents, aqueous solubility does not guarantee decreased π-stacking or aggregation. The resulting broadening of the absorbance spectrum is a detriment to photoacoustic (PA) imaging since the signal intensity, accuracy, and image quality all rely on reliable spectral unmixing. To address this major limitation and further enhance the tumor-targeting ability of imaging agents, we have taken a biomimetic approach to design a multivalent glucose moiety (mvGlu). We showcase the utility of this new group by developing aza-BODIPY-based contrast agents boasting a significant PA signal enhancement greater than 11-fold after spectral unmixing. Moreover, when applied to targeting cancer cells, effective staining could be achieved with ultra-low dye concentrations (50 nM) and compared to a non-targeted analogue, the signal intensity was >1000-fold higher. Lastly, we employed the mvGlu technology to develop a logic-gated acoustogenic probe to detect intratumoral copper (i.e., Cu(I)), which is an emerging cancer biomarker, in a murine model of breast cancer. This exciting application was not possible using other acoustogenic probes previously developed for copper sensing.
AB - The attachment of glucose to drugs and imaging agents enables cancer cell targeting via interactions with GLUT1 overexpressed on the cell surface. While an added benefit of this modification is the solubilizing effect of carbohydrates, in the context of imaging agents, aqueous solubility does not guarantee decreased π-stacking or aggregation. The resulting broadening of the absorbance spectrum is a detriment to photoacoustic (PA) imaging since the signal intensity, accuracy, and image quality all rely on reliable spectral unmixing. To address this major limitation and further enhance the tumor-targeting ability of imaging agents, we have taken a biomimetic approach to design a multivalent glucose moiety (mvGlu). We showcase the utility of this new group by developing aza-BODIPY-based contrast agents boasting a significant PA signal enhancement greater than 11-fold after spectral unmixing. Moreover, when applied to targeting cancer cells, effective staining could be achieved with ultra-low dye concentrations (50 nM) and compared to a non-targeted analogue, the signal intensity was >1000-fold higher. Lastly, we employed the mvGlu technology to develop a logic-gated acoustogenic probe to detect intratumoral copper (i.e., Cu(I)), which is an emerging cancer biomarker, in a murine model of breast cancer. This exciting application was not possible using other acoustogenic probes previously developed for copper sensing.
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U2 - 10.1021/jacs.2c13489
DO - 10.1021/jacs.2c13489
M3 - Article
C2 - 36973171
AN - SCOPUS:85151342527
SN - 0002-7863
VL - 145
SP - 7313
EP - 7322
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
IS - 13
ER -