TY - JOUR
T1 - Biological controls for standardization and interpretation of adaptive immune receptor repertoire profiling
AU - AIRR Community
AU - Trück, Johannes
AU - Eugster, Anne
AU - Barennes, Pierre
AU - Tipton, Christopher M.
AU - Luning Prak, Eline T.
AU - Bagnara, Davide
AU - Soto, Cinque
AU - Sherkow, Jacob S.
AU - Payne, Aimee S.
AU - Lefranc, Marie Paule
AU - Farmer, Andrew
AU - Bostick, Magnolia
AU - Mariotti-Ferrandiz, Encarnita
N1 - Publisher Copyright:
© 2021, Trück et al.
PY - 2021/5/26
Y1 - 2021/5/26
N2 - Use of adaptive immune receptor repertoire sequencing (AIRR-seq) has become widespread, providing new insights into the immune system with potential broad clinical and diagnostic applications. However, like many high-throughput technologies, it comes with several problems, and the AIRR Community was established to understand and help solve them. We, the AIRR Community's Biological Resources Working Group, have surveyed scientists about the need for standards and controls in generating and annotating AIRR-seq data. Here, we review the current status of AIRR-seq, provide the results of our survey, and based on them, offer recommendations for developing AIRR-seq standards and controls, including future work.
AB - Use of adaptive immune receptor repertoire sequencing (AIRR-seq) has become widespread, providing new insights into the immune system with potential broad clinical and diagnostic applications. However, like many high-throughput technologies, it comes with several problems, and the AIRR Community was established to understand and help solve them. We, the AIRR Community's Biological Resources Working Group, have surveyed scientists about the need for standards and controls in generating and annotating AIRR-seq data. Here, we review the current status of AIRR-seq, provide the results of our survey, and based on them, offer recommendations for developing AIRR-seq standards and controls, including future work.
KW - antibody
KW - B-cell Receptor (BCR)
KW - IG
KW - immunoglobulin
KW - immunology
KW - inflammation
KW - next generation sequencing (NGS)
KW - T-cell Receptor (TCR)
KW - TR
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U2 - 10.7554/eLife.66274
DO - 10.7554/eLife.66274
M3 - Article
C2 - 34037521
AN - SCOPUS:85107085503
VL - 10
JO - eLife
JF - eLife
SN - 2050-084X
M1 - e66274
ER -