Bioinspired glycosaminoglycan hydrogels via click chemistry for 3D dynamic cell encapsulation

Liangju Kuang, Nur P. Damayanti, Chunhui Jiang, Xing Fei, Wenjie Liu, Naagarajan Narayanan, Joseph Maria Kumar Irudayaraj, Osvaldo Campanella, Meng Deng

Research output: Contribution to journalArticle

Abstract

Cell encapsulation within 3D hydrogels is an attractive approach to develop effective cell-based therapies. However, little is known about how cells respond to the dynamic microenvironment resulting from hydrogel gelation-based cell encapsulation. Here, a tunable biomimetic hydrogel system that possesses alterable gelation kinetics and biologically relevant matrix stiffness is developed to study 3D dynamic cellular responses during encapsulation. Hydrogels are synthesized by crosslinking thiolated hyaluronic acid and thiolated chondroitin sulfate with poly(ethylene glycol) diacrylate under cell-compatible conditions. Hydrogel properties are tailored by altering thiol substitution degrees of glycosaminoglycans or molecular weights of crosslinkers. Encapsulation of human mesenchymal stem cells through hydrogel gelation reveals high cell viability as well as a three-stage gelation-dependent cellular response in real-time focal adhesion kinase (FAK) phosphorylation in live single cells. Furthermore, stiffer hydrogels result in higher equilibrium FAK activity and enhanced actin protrusions. Our results demonstrate the promise of hydrogel-mediated cellular responses during cell encapsulation.

LanguageEnglish (US)
Article number47212
JournalJournal of Applied Polymer Science
Volume136
Issue number5
DOIs
StatePublished - Feb 5 2019

Fingerprint

Hyaluronic acid
Sulfur Compounds
Focal Adhesion Protein-Tyrosine Kinases
Hydrogels
Sulfur compounds
Hydrogel
Biomimetics
Stiffness matrix
Gelation
Hyaluronic Acid
Glycosaminoglycans
Stem cells
Encapsulation
Cell culture
Crosslinking
Polyethylene glycols
Adhesion
Enzymes
Phosphorylation
Chondroitin Sulfates

Keywords

  • cell encapsulation
  • focal adhesion kinase
  • glycosaminoglycans
  • human mesenchymal stem cells
  • hydrogels

ASJC Scopus subject areas

  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Kuang, L., Damayanti, N. P., Jiang, C., Fei, X., Liu, W., Narayanan, N., ... Deng, M. (2019). Bioinspired glycosaminoglycan hydrogels via click chemistry for 3D dynamic cell encapsulation. Journal of Applied Polymer Science, 136(5), [47212]. https://doi.org/10.1002/app.47212

Bioinspired glycosaminoglycan hydrogels via click chemistry for 3D dynamic cell encapsulation. / Kuang, Liangju; Damayanti, Nur P.; Jiang, Chunhui; Fei, Xing; Liu, Wenjie; Narayanan, Naagarajan; Irudayaraj, Joseph Maria Kumar; Campanella, Osvaldo; Deng, Meng.

In: Journal of Applied Polymer Science, Vol. 136, No. 5, 47212, 05.02.2019.

Research output: Contribution to journalArticle

Kuang, L, Damayanti, NP, Jiang, C, Fei, X, Liu, W, Narayanan, N, Irudayaraj, JMK, Campanella, O & Deng, M 2019, 'Bioinspired glycosaminoglycan hydrogels via click chemistry for 3D dynamic cell encapsulation' Journal of Applied Polymer Science, vol. 136, no. 5, 47212. https://doi.org/10.1002/app.47212
Kuang, Liangju ; Damayanti, Nur P. ; Jiang, Chunhui ; Fei, Xing ; Liu, Wenjie ; Narayanan, Naagarajan ; Irudayaraj, Joseph Maria Kumar ; Campanella, Osvaldo ; Deng, Meng. / Bioinspired glycosaminoglycan hydrogels via click chemistry for 3D dynamic cell encapsulation. In: Journal of Applied Polymer Science. 2019 ; Vol. 136, No. 5.
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