Abstract

Neuropeptides and peptide hormones are signaling molecules produced via complex post-translational modifications of precursor proteins known as prohormones. Neuropeptides activate specific receptors and are associated with the regulation of physiological systems and behaviors. The identification of prohormones—and the neuropeptides created by these prohormones—from genomic assemblies has become essential to support the annotation and use of the rapidly growing number of sequenced genomes. Here we describe a methodology for identifying the prohormone complement from genomic assemblies that employs widely available public toolsets and databases. The uncovered prohormone sequences can then be screened for putative neuropeptides to enable accurate proteomic discovery and validation.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages71-96
Number of pages26
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1719
ISSN (Print)1064-3745

Fingerprint

Computational Biology
Neuropeptides
Peptide Hormones
Post Translational Protein Processing
Proteomics
Genome
Databases

Keywords

  • Bioinformatics
  • Cleavage
  • Gene prediction
  • Homology
  • Neuropeptide
  • Prohormone

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Southey, B. R., Romanova, E. V., Rodriguez-Zas, S. L., & Sweedler, J. V. (2018). Bioinformatics for prohormone and neuropeptide discovery. In Methods in Molecular Biology (pp. 71-96). (Methods in Molecular Biology; Vol. 1719). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7537-2_5

Bioinformatics for prohormone and neuropeptide discovery. / Southey, Bruce R.; Romanova, Elena Vikentievna; Rodriguez-Zas, Sandra Luisa; Sweedler, Jonathan V.

Methods in Molecular Biology. Humana Press Inc., 2018. p. 71-96 (Methods in Molecular Biology; Vol. 1719).

Research output: Chapter in Book/Report/Conference proceedingChapter

Southey, BR, Romanova, EV, Rodriguez-Zas, SL & Sweedler, JV 2018, Bioinformatics for prohormone and neuropeptide discovery. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1719, Humana Press Inc., pp. 71-96. https://doi.org/10.1007/978-1-4939-7537-2_5
Southey BR, Romanova EV, Rodriguez-Zas SL, Sweedler JV. Bioinformatics for prohormone and neuropeptide discovery. In Methods in Molecular Biology. Humana Press Inc. 2018. p. 71-96. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7537-2_5
Southey, Bruce R. ; Romanova, Elena Vikentievna ; Rodriguez-Zas, Sandra Luisa ; Sweedler, Jonathan V. / Bioinformatics for prohormone and neuropeptide discovery. Methods in Molecular Biology. Humana Press Inc., 2018. pp. 71-96 (Methods in Molecular Biology).
@inbook{73e8857c30274d6a8caff559e5371a72,
title = "Bioinformatics for prohormone and neuropeptide discovery",
abstract = "Neuropeptides and peptide hormones are signaling molecules produced via complex post-translational modifications of precursor proteins known as prohormones. Neuropeptides activate specific receptors and are associated with the regulation of physiological systems and behaviors. The identification of prohormones—and the neuropeptides created by these prohormones—from genomic assemblies has become essential to support the annotation and use of the rapidly growing number of sequenced genomes. Here we describe a methodology for identifying the prohormone complement from genomic assemblies that employs widely available public toolsets and databases. The uncovered prohormone sequences can then be screened for putative neuropeptides to enable accurate proteomic discovery and validation.",
keywords = "Bioinformatics, Cleavage, Gene prediction, Homology, Neuropeptide, Prohormone",
author = "Southey, {Bruce R.} and Romanova, {Elena Vikentievna} and Rodriguez-Zas, {Sandra Luisa} and Sweedler, {Jonathan V}",
year = "2018",
month = "1",
day = "1",
doi = "10.1007/978-1-4939-7537-2_5",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "71--96",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Bioinformatics for prohormone and neuropeptide discovery

AU - Southey, Bruce R.

AU - Romanova, Elena Vikentievna

AU - Rodriguez-Zas, Sandra Luisa

AU - Sweedler, Jonathan V

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Neuropeptides and peptide hormones are signaling molecules produced via complex post-translational modifications of precursor proteins known as prohormones. Neuropeptides activate specific receptors and are associated with the regulation of physiological systems and behaviors. The identification of prohormones—and the neuropeptides created by these prohormones—from genomic assemblies has become essential to support the annotation and use of the rapidly growing number of sequenced genomes. Here we describe a methodology for identifying the prohormone complement from genomic assemblies that employs widely available public toolsets and databases. The uncovered prohormone sequences can then be screened for putative neuropeptides to enable accurate proteomic discovery and validation.

AB - Neuropeptides and peptide hormones are signaling molecules produced via complex post-translational modifications of precursor proteins known as prohormones. Neuropeptides activate specific receptors and are associated with the regulation of physiological systems and behaviors. The identification of prohormones—and the neuropeptides created by these prohormones—from genomic assemblies has become essential to support the annotation and use of the rapidly growing number of sequenced genomes. Here we describe a methodology for identifying the prohormone complement from genomic assemblies that employs widely available public toolsets and databases. The uncovered prohormone sequences can then be screened for putative neuropeptides to enable accurate proteomic discovery and validation.

KW - Bioinformatics

KW - Cleavage

KW - Gene prediction

KW - Homology

KW - Neuropeptide

KW - Prohormone

UR - http://www.scopus.com/inward/record.url?scp=85042561817&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85042561817&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-7537-2_5

DO - 10.1007/978-1-4939-7537-2_5

M3 - Chapter

T3 - Methods in Molecular Biology

SP - 71

EP - 96

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -