Bioethanol Production from Lignocellulosics: Some Process Considerations and Procedures

Lucas Loveless, Wu Li Bao, Kyle E. Beery, Martin Sparks, Consuelo Cruz, Charles A. Abbas, Pamela Corrington, Kelli Trei

Research output: Chapter in Book/Report/Conference proceedingOther chapter contribution


The current emphasis on environmentally friendly liquid transportation fuels has caused a renewed interest in bioethanol and, in particular, lignocellulosic feedstocks for the production of bioethanol. As a result, research into the development of an economic process for the bioconversion of lignocellulosics has accelerated. The primary areas of research crucial to improved process economics consist of feedstock selection, pretreatment, hydrolysis of the pretreated feedstocks, and developing an optimized fermentation process that uses newly engineered ethanologens capable of utilizing various biomass-derived sugars. Various levels of cellulose, hemicellulose, lignin, ash, silica, nitrogen, and moisture will require various pretreatment conditions and chemicals to ensure the most optimum pretreatment for that feedstock. The various pretreatments range from seconds for dilute acid, ammonia fiber explosion (AFEX), and steam explosion to days and weeks for lime pretreatment. Acid pretreatments include both processes featuring the addition of acids and processes without added acids in which the pH is lowered due to release of acetic acid in the course of pretreatment. Pretreatments using bases, such as AFEX, lime pretreatment, and ammonia recycle percolation alone or followed by a successive treatment with hydrogen peroxide, work mainly by swelling the biomass and modifying or solubilizing the hemicellulose and lignin. The enzyme products can be analyzed on silica thin-layer chromatography plates, where sugars are visualized by sulfuric acid charring at high temperature. Lignocellulosic hydrolysate fermentation or bioconversion may be achieved by either solid-state or submerged-liquid fermentation.
Original languageEnglish (US)
Title of host publicationManual of Industrial Microbiology and Biotechnology, Third Edition
EditorsLee R. Lynd, Huimin Zhao, Leonard Katz, Richard H. Baltz, Alan T. Bull, Beth Junker, Prakash Masurekar, Julian E. Davies, Christopher D. Reeves, Arnold L. Demain
PublisherAmerican Society of Microbiology
ISBN (Electronic)9781555816827
ISBN (Print)9781555815127
StatePublished - Jan 1 2010

Fingerprint Dive into the research topics of 'Bioethanol Production from Lignocellulosics: Some Process Considerations and Procedures'. Together they form a unique fingerprint.

Cite this