Bioethanol production from cellulosic hydrolysates by engineered industrial Saccharomyces cerevisiae

Ye Gi Lee, Yong Su Jin, Young Lok Cha, Jin Ho Seo

Research output: Contribution to journalArticlepeer-review


Even though industrial yeast strains exhibit numerous advantageous traits for the production of bioethanol, their genetic manipulation has been limited. This study demonstrates that an industrial polyploidy Saccharomyces cerevisiae JHS200 can be engineered through Cas9 (CRISPR associated protein 9)-based genome editing. Specifically, we generated auxotrophic mutants and introduced a xylose metabolic pathway into the auxotrophic mutants. As expected, the engineered strain (JX123) enhanced ethanol production from cellulosic hydrolysates as compared to other engineered haploid strains. However, the JX123 strain produced substantial amounts of xylitol as a by-product during xylose fermentation. Hypothesizing that the xylitol accumulation might be caused by intracellular redox imbalance from cofactor difference, the NADH oxidase from Lactococcus lactis was introduced into the JX123 strain. The resulting strain (JX123_noxE) not only produced more ethanol, but also produced xylitol less than the JX123 strain. These results suggest that industrial polyploidy yeast can be modified for producing biofuels and chemicals.

Original languageEnglish (US)
Pages (from-to)355-361
Number of pages7
JournalBioresource Technology
StatePublished - 2017


  • Cas9 genome editing
  • Hydrolysate fermentation
  • Industrial Saccharomyces cerevisiae
  • Lignocellulosic ethanol

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


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