TY - JOUR
T1 - Bioengineering methods for analysis of cells in vitro
AU - Underhill, Gregory H.
AU - Peter, Galie
AU - Chen, Christopher S.
AU - Bhatia, Sangeeta N.
PY - 2012
Y1 - 2012
N2 - Efforts in the interdisciplinary field of bioengineering have led to innovative methods for investigating the complexities of cell responses in vitro. These approaches have emphasized the reduction of complex multicomponent cellular microenvironments into distinct individual signals as a means to both (a) better construct mimics of in vivo microenvironments and (b) better deconstruct microenvironments to study them. Microtechnology tools, together with advances in biomaterials, have been fundamental to this progress by enabling the tightly controlled presentation of environmental cues and the improved systematic analysis of cellular perturbations. In this review, we describe bioengineering approaches for controlling and measuring cell-environmental interactions in vitro, including strategies for high-throughput analysis. We also describe the mechanistic insights gained by the use of these novel tools, with associated applications ranging from fundamental biological studies, in vitro modeling of in vivo processes, and cell-based therapies.
AB - Efforts in the interdisciplinary field of bioengineering have led to innovative methods for investigating the complexities of cell responses in vitro. These approaches have emphasized the reduction of complex multicomponent cellular microenvironments into distinct individual signals as a means to both (a) better construct mimics of in vivo microenvironments and (b) better deconstruct microenvironments to study them. Microtechnology tools, together with advances in biomaterials, have been fundamental to this progress by enabling the tightly controlled presentation of environmental cues and the improved systematic analysis of cellular perturbations. In this review, we describe bioengineering approaches for controlling and measuring cell-environmental interactions in vitro, including strategies for high-throughput analysis. We also describe the mechanistic insights gained by the use of these novel tools, with associated applications ranging from fundamental biological studies, in vitro modeling of in vivo processes, and cell-based therapies.
KW - biomaterial
KW - extracellular matrix
KW - high-throughput
KW - mechanobiology
KW - microfabrication
UR - http://www.scopus.com/inward/record.url?scp=84870163822&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84870163822&partnerID=8YFLogxK
U2 - 10.1146/annurev-cellbio-101011-155709
DO - 10.1146/annurev-cellbio-101011-155709
M3 - Review article
C2 - 23057744
AN - SCOPUS:84870163822
SN - 1081-0706
VL - 28
SP - 385
EP - 410
JO - Annual review of cell and developmental biology
JF - Annual review of cell and developmental biology
ER -