Bioconversion of (R)-(+)-limonene by P. digitatum (NRRL 1202)

Q. Tan, D. F. Day, K. R. Cadwallader

Research output: Contribution to journalArticlepeer-review

Abstract

Racemic limonene was enantio-selectively and enantio-specifically converted to (R)-(+)-α-terpineol by Penicillium digitatum mycelia. Bioconversion only occurred during the early to mid-log phase of growth. This pattern held true when different age inocula were used. Bioconversion activity increased more than 4-fold after substrate induction. Aeration was critical to this bioconversion. The optimum oxygen requirement was 1.6 mg/litre. Up to 4% (v/v) limonene did not inhibit the bioconversion. α- Terpineol inhibition was seen at concentrations greater than 25.9 mM. The optimum conversion temperature was 28°C, and the optimum pH was 4.5. The highest concentration of product was formed with a contact time of between 1 and 2 days.

Original languageEnglish (US)
Pages (from-to)29-37
Number of pages9
JournalProcess Biochemistry
Volume33
Issue number1
DOIs
StatePublished - 1998
Externally publishedYes

Keywords

  • Bioconversion
  • Flavours
  • Limonene
  • P. Digitatum
  • Substrate induction
  • α-terpined

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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