Bioconversion of (R)-(+)-limonene by P. digitatum (NRRL 1202)

Q. Tan; D. F. Day; K. R. Cadwallader

doi:10.1016/S0032-9592(97)00048-4

Bioconversion of (R)-(+)-limonene by P. digitatum (NRRL 1202)

Research output: Contribution to journal › Article › peer-review

Abstract

Racemic limonene was enantio-selectively and enantio-specifically converted to (R)-(+)-α-terpineol by Penicillium digitatum mycelia. Bioconversion only occurred during the early to mid-log phase of growth. This pattern held true when different age inocula were used. Bioconversion activity increased more than 4-fold after substrate induction. Aeration was critical to this bioconversion. The optimum oxygen requirement was 1.6 mg/litre. Up to 4% (v/v) limonene did not inhibit the bioconversion. α- Terpineol inhibition was seen at concentrations greater than 25.9 mM. The optimum conversion temperature was 28°C, and the optimum pH was 4.5. The highest concentration of product was formed with a contact time of between 1 and 2 days.

Original language	English (US)
Pages (from-to)	29-37
Number of pages	9
Journal	Process Biochemistry
Volume	33
Issue number	1
DOIs	https://doi.org/10.1016/S0032-9592(97)00048-4
State	Published - 1998
Externally published	Yes

Keywords

Bioconversion
Flavours
Limonene
P. Digitatum
Substrate induction
α-terpined

ASJC Scopus subject areas

Bioengineering
Biochemistry
Applied Microbiology and Biotechnology

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title = "Bioconversion of (R)-(+)-limonene by P. digitatum (NRRL 1202)",

abstract = "Racemic limonene was enantio-selectively and enantio-specifically converted to (R)-(+)-α-terpineol by Penicillium digitatum mycelia. Bioconversion only occurred during the early to mid-log phase of growth. This pattern held true when different age inocula were used. Bioconversion activity increased more than 4-fold after substrate induction. Aeration was critical to this bioconversion. The optimum oxygen requirement was 1.6 mg/litre. Up to 4% (v/v) limonene did not inhibit the bioconversion. α- Terpineol inhibition was seen at concentrations greater than 25.9 mM. The optimum conversion temperature was 28°C, and the optimum pH was 4.5. The highest concentration of product was formed with a contact time of between 1 and 2 days.",

keywords = "Bioconversion, Flavours, Limonene, P. Digitatum, Substrate induction, α-terpined",

author = "Q. Tan and Day, {D. F.} and Cadwallader, {K. R.}",

year = "1998",

doi = "10.1016/S0032-9592(97)00048-4",

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volume = "33",

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T1 - Bioconversion of (R)-(+)-limonene by P. digitatum (NRRL 1202)

AU - Tan, Q.

AU - Day, D. F.

AU - Cadwallader, K. R.

PY - 1998

Y1 - 1998

N2 - Racemic limonene was enantio-selectively and enantio-specifically converted to (R)-(+)-α-terpineol by Penicillium digitatum mycelia. Bioconversion only occurred during the early to mid-log phase of growth. This pattern held true when different age inocula were used. Bioconversion activity increased more than 4-fold after substrate induction. Aeration was critical to this bioconversion. The optimum oxygen requirement was 1.6 mg/litre. Up to 4% (v/v) limonene did not inhibit the bioconversion. α- Terpineol inhibition was seen at concentrations greater than 25.9 mM. The optimum conversion temperature was 28°C, and the optimum pH was 4.5. The highest concentration of product was formed with a contact time of between 1 and 2 days.

AB - Racemic limonene was enantio-selectively and enantio-specifically converted to (R)-(+)-α-terpineol by Penicillium digitatum mycelia. Bioconversion only occurred during the early to mid-log phase of growth. This pattern held true when different age inocula were used. Bioconversion activity increased more than 4-fold after substrate induction. Aeration was critical to this bioconversion. The optimum oxygen requirement was 1.6 mg/litre. Up to 4% (v/v) limonene did not inhibit the bioconversion. α- Terpineol inhibition was seen at concentrations greater than 25.9 mM. The optimum conversion temperature was 28°C, and the optimum pH was 4.5. The highest concentration of product was formed with a contact time of between 1 and 2 days.

KW - Bioconversion

KW - Flavours

KW - Limonene

KW - P. Digitatum

KW - Substrate induction

KW - α-terpined

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ER -

Bioconversion of (R)-(+)-limonene by P. digitatum (NRRL 1202)

Abstract

Keywords

ASJC Scopus subject areas

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