Abstract
Racemic limonene was enantio-selectively and enantio-specifically converted to (R)-(+)-α-terpineol by Penicillium digitatum mycelia. Bioconversion only occurred during the early to mid-log phase of growth. This pattern held true when different age inocula were used. Bioconversion activity increased more than 4-fold after substrate induction. Aeration was critical to this bioconversion. The optimum oxygen requirement was 1.6 mg/litre. Up to 4% (v/v) limonene did not inhibit the bioconversion. α- Terpineol inhibition was seen at concentrations greater than 25.9 mM. The optimum conversion temperature was 28°C, and the optimum pH was 4.5. The highest concentration of product was formed with a contact time of between 1 and 2 days.
Original language | English (US) |
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Pages (from-to) | 29-37 |
Number of pages | 9 |
Journal | Process Biochemistry |
Volume | 33 |
Issue number | 1 |
DOIs | |
State | Published - 1998 |
Externally published | Yes |
Keywords
- Bioconversion
- Flavours
- Limonene
- P. Digitatum
- Substrate induction
- α-terpined
ASJC Scopus subject areas
- Bioengineering
- Biochemistry
- Applied Microbiology and Biotechnology