Bioconversion of (+)‐Limonene by Pseudomonas gladioli

Pseudomonas gladioli was isolated by an enrichment culture technique using limonene as the sole source of carbon. Fermentations were conducted for 4–10 days in shake flasks at 25°C using a pH 6.5 mineral salts medium and 1.0% (+)‐limonene. Major conversion products were identified as (+)‐α‐terpineol and (+)‐perillic acid. Quantitative analysis of α‐terpineol and perillic acid during the fermentation revealed that α‐terpineol was resistant to further degradation by the bacterium, while perillic acid was readily degraded.