Bioconversion of α- and β-pinene by Pseudomonas sp. strain PIN

S. K. Yoo, D. F. Day, K. R. Cadwallader

Research output: Contribution to journalArticlepeer-review

Abstract

A soil pseudomonad capable of metabolizing either α- or β-pinene as sole carbon and energy source was isolated with an enrichment culture. Culture broth in mineral media gave rise to a pine-like aroma during the initial fermentation stage and a fresh rose-like aroma during the late fermentation stage. No growth inhibition was found up to 10% pinenes concentration. The bioconversion products of α-pinene were identified as limonene, p-cymene, α-terpinolene, camphor, terpinen-4-ol, α-terpineol, endo-borneol, and p-cymene-8-ol. The bioconversion products of β-pinene were the same except for the presence of fenchyl alcohol and the absence of camphor. Most of the accumulated products were p-menthene derivatives such as p-cymene, limonene, and α-terpinolene in both substrates. Total bioconversion was 33.5% of α-pinene and 58.8% of β-pinene.

Original languageEnglish (US)
Pages (from-to)925-932
Number of pages8
JournalProcess Biochemistry
Volume36
Issue number10
DOIs
StatePublished - May 26 2001
Externally publishedYes

Keywords

  • Bioconversion
  • Flavours
  • Monoterpenes
  • Substrate concentration
  • α-pinene
  • β-pinene

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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