Biocompatibility and biodistribution of surface-enhanced raman scattering nanoprobes in zebrafish embryos: In vivo and multiplex imaging

Yuling Wang, Jamie L. Seebald, Daniel P. Szeto, Joseph Irudayaraj

Research output: Contribution to journalArticle

Abstract

Nanoparticles are increasingly being used to investigate biological processes in various animal models due to their versatile chemical, unique optical, and multifunctional properties. In this report we address the biocompatibility and biodistribution of nanoparticle sensors used for Raman chemical imaging in live zebrafish (Danio rerio) embryos. Surface-enhanced Raman scattering (SERS) nanoprobes (NPs) comprising gold nanoparticles (AuNPs) as enhancing substrate and nonfluorescent Raman labels were synthesized and microinjected into zebrafish embryos at the one-cell stage. Raman mapping was performed to assess their distribution in various cell-types and tissues of developing embryo at five different stages between 6 and 96 hpf (hours post-fertilization). Biocompatibility and toxicity studies indicate that the NPs are not toxic and the embryos were found to exhibit normal morphological and gene expression in addition to the proper form and function of major organs such as the heart and vasculature (of 7 day old NPs injected zebrafish embryos). A multiplex in vivo detection protocol was developed by SERS imaging to demonstrate that multiple labels can be detected by Raman mapping in undifferentiated cells as they develop into distinct cell- and tissue-types. The present work is the first to report on multiplex Raman imaging of zebrafish embryos with potential implications in tracking tissue development and biological processes at single molecule sensitivity using appropriate target molecules in vivo.

Original languageEnglish (US)
Pages (from-to)4039-4053
Number of pages15
JournalACS Nano
Volume4
Issue number7
DOIs
StatePublished - Jul 27 2010

Fingerprint

Nanoprobes
embryos
biocompatibility
Biocompatibility
Raman scattering
Tissue
Raman spectra
Nanoparticles
Imaging techniques
Labels
Molecules
Poisons
cells
Gene expression
Gold
nanoparticles
Toxicity
Animals
fertilization
animal models

Keywords

  • SERS nanoprobes
  • biocompatibility
  • biodistribution
  • gene expression
  • multiplex in vivo Raman mapping
  • zebrafish embryos

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Biocompatibility and biodistribution of surface-enhanced raman scattering nanoprobes in zebrafish embryos : In vivo and multiplex imaging. / Wang, Yuling; Seebald, Jamie L.; Szeto, Daniel P.; Irudayaraj, Joseph.

In: ACS Nano, Vol. 4, No. 7, 27.07.2010, p. 4039-4053.

Research output: Contribution to journalArticle

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