Biochemical basis of the activation of promutagens by plant cell systems

Michael Jacob Plewa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Plant activation is the process by which promutagenic agents are activated into mutagens by plant systems. Many promutagens are activated by plants as well as by the familiar mammalian microsomal monooxygenase systems. However, several environmentally important agents are preferentially activated by plant cells. Plants have become a reservoir for the deposition and accumulation of environmental xenobiotics. With the widespread use of agricultural chemicals on crop plants and with the global exposure of plants to pollutants, the possibility that plant-activated agents may be introduced into the human food chain is a cause of concern. Environmentally relevant agents should be evaluated with plant assays. The plant cell/microbe coincubation assay uses cultured plant cell suspensions as the activating system and bacteria or yeast cells as the genetic indicator organism. After a treatment time, the microbes are plated on selective medium. In this way the activation system and the genetic system can be independently studied. In addition, the viability of the plant cells and the microbial cells can be independently determined so that the toxicity of a test agent can be evaluated. We have employed cultured tobacco, cotton, carrot, maize, and Tradescantia cells to study the activation of test agents and complex environmental mixtures. In addition to screening, this assay is being used in basic research to elucidate the biochemical mechanisms of plant activation. The results of experiments using the peroxidase inhibitors acetaminophen and diethyldithiocarbamate showing repression of TX1-cell activation of m-phenylenediamine and 2-aminofluorene indicate that a TX1-cell peroxidase pathway is involved in the plant activation of aromatic amines.

Original languageEnglish (US)
Title of host publicationASTM Special Technical Publication
EditorsJoseph W. Gorsuch, William R. Lower, Michael A. Lewis, Shahbeg Sandhu, Wuncheng Wang
PublisherPubl by ASTM
Pages287-296
Number of pages10
Edition1115
ISBN (Print)0803114222
StatePublished - 1991
Event2nd Symposium on Use of Plants for Toxicity Assessment - San Francisco, CA, USA
Duration: Apr 23 1990Apr 24 1990

Other

Other2nd Symposium on Use of Plants for Toxicity Assessment
CitySan Francisco, CA, USA
Period4/23/904/24/90

Fingerprint

Chemical activation
Assays
Agricultural chemicals
Mutagens
Tobacco
Yeast
Cotton
Crops
Toxicity
Plant Cells
Amines
Bacteria
Screening
Cells
Experiments

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Plewa, M. J. (1991). Biochemical basis of the activation of promutagens by plant cell systems. In J. W. Gorsuch, W. R. Lower, M. A. Lewis, S. Sandhu, & W. Wang (Eds.), ASTM Special Technical Publication (1115 ed., pp. 287-296). Publ by ASTM.

Biochemical basis of the activation of promutagens by plant cell systems. / Plewa, Michael Jacob.

ASTM Special Technical Publication. ed. / Joseph W. Gorsuch; William R. Lower; Michael A. Lewis; Shahbeg Sandhu; Wuncheng Wang. 1115. ed. Publ by ASTM, 1991. p. 287-296.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Plewa, MJ 1991, Biochemical basis of the activation of promutagens by plant cell systems. in JW Gorsuch, WR Lower, MA Lewis, S Sandhu & W Wang (eds), ASTM Special Technical Publication. 1115 edn, Publ by ASTM, pp. 287-296, 2nd Symposium on Use of Plants for Toxicity Assessment, San Francisco, CA, USA, 4/23/90.
Plewa MJ. Biochemical basis of the activation of promutagens by plant cell systems. In Gorsuch JW, Lower WR, Lewis MA, Sandhu S, Wang W, editors, ASTM Special Technical Publication. 1115 ed. Publ by ASTM. 1991. p. 287-296
Plewa, Michael Jacob. / Biochemical basis of the activation of promutagens by plant cell systems. ASTM Special Technical Publication. editor / Joseph W. Gorsuch ; William R. Lower ; Michael A. Lewis ; Shahbeg Sandhu ; Wuncheng Wang. 1115. ed. Publ by ASTM, 1991. pp. 287-296
@inproceedings{633515896c8e4ba4a98da68103817888,
title = "Biochemical basis of the activation of promutagens by plant cell systems",
abstract = "Plant activation is the process by which promutagenic agents are activated into mutagens by plant systems. Many promutagens are activated by plants as well as by the familiar mammalian microsomal monooxygenase systems. However, several environmentally important agents are preferentially activated by plant cells. Plants have become a reservoir for the deposition and accumulation of environmental xenobiotics. With the widespread use of agricultural chemicals on crop plants and with the global exposure of plants to pollutants, the possibility that plant-activated agents may be introduced into the human food chain is a cause of concern. Environmentally relevant agents should be evaluated with plant assays. The plant cell/microbe coincubation assay uses cultured plant cell suspensions as the activating system and bacteria or yeast cells as the genetic indicator organism. After a treatment time, the microbes are plated on selective medium. In this way the activation system and the genetic system can be independently studied. In addition, the viability of the plant cells and the microbial cells can be independently determined so that the toxicity of a test agent can be evaluated. We have employed cultured tobacco, cotton, carrot, maize, and Tradescantia cells to study the activation of test agents and complex environmental mixtures. In addition to screening, this assay is being used in basic research to elucidate the biochemical mechanisms of plant activation. The results of experiments using the peroxidase inhibitors acetaminophen and diethyldithiocarbamate showing repression of TX1-cell activation of m-phenylenediamine and 2-aminofluorene indicate that a TX1-cell peroxidase pathway is involved in the plant activation of aromatic amines.",
author = "Plewa, {Michael Jacob}",
year = "1991",
language = "English (US)",
isbn = "0803114222",
pages = "287--296",
editor = "Gorsuch, {Joseph W.} and Lower, {William R.} and Lewis, {Michael A.} and Shahbeg Sandhu and Wuncheng Wang",
booktitle = "ASTM Special Technical Publication",
publisher = "Publ by ASTM",
edition = "1115",

}

TY - GEN

T1 - Biochemical basis of the activation of promutagens by plant cell systems

AU - Plewa, Michael Jacob

PY - 1991

Y1 - 1991

N2 - Plant activation is the process by which promutagenic agents are activated into mutagens by plant systems. Many promutagens are activated by plants as well as by the familiar mammalian microsomal monooxygenase systems. However, several environmentally important agents are preferentially activated by plant cells. Plants have become a reservoir for the deposition and accumulation of environmental xenobiotics. With the widespread use of agricultural chemicals on crop plants and with the global exposure of plants to pollutants, the possibility that plant-activated agents may be introduced into the human food chain is a cause of concern. Environmentally relevant agents should be evaluated with plant assays. The plant cell/microbe coincubation assay uses cultured plant cell suspensions as the activating system and bacteria or yeast cells as the genetic indicator organism. After a treatment time, the microbes are plated on selective medium. In this way the activation system and the genetic system can be independently studied. In addition, the viability of the plant cells and the microbial cells can be independently determined so that the toxicity of a test agent can be evaluated. We have employed cultured tobacco, cotton, carrot, maize, and Tradescantia cells to study the activation of test agents and complex environmental mixtures. In addition to screening, this assay is being used in basic research to elucidate the biochemical mechanisms of plant activation. The results of experiments using the peroxidase inhibitors acetaminophen and diethyldithiocarbamate showing repression of TX1-cell activation of m-phenylenediamine and 2-aminofluorene indicate that a TX1-cell peroxidase pathway is involved in the plant activation of aromatic amines.

AB - Plant activation is the process by which promutagenic agents are activated into mutagens by plant systems. Many promutagens are activated by plants as well as by the familiar mammalian microsomal monooxygenase systems. However, several environmentally important agents are preferentially activated by plant cells. Plants have become a reservoir for the deposition and accumulation of environmental xenobiotics. With the widespread use of agricultural chemicals on crop plants and with the global exposure of plants to pollutants, the possibility that plant-activated agents may be introduced into the human food chain is a cause of concern. Environmentally relevant agents should be evaluated with plant assays. The plant cell/microbe coincubation assay uses cultured plant cell suspensions as the activating system and bacteria or yeast cells as the genetic indicator organism. After a treatment time, the microbes are plated on selective medium. In this way the activation system and the genetic system can be independently studied. In addition, the viability of the plant cells and the microbial cells can be independently determined so that the toxicity of a test agent can be evaluated. We have employed cultured tobacco, cotton, carrot, maize, and Tradescantia cells to study the activation of test agents and complex environmental mixtures. In addition to screening, this assay is being used in basic research to elucidate the biochemical mechanisms of plant activation. The results of experiments using the peroxidase inhibitors acetaminophen and diethyldithiocarbamate showing repression of TX1-cell activation of m-phenylenediamine and 2-aminofluorene indicate that a TX1-cell peroxidase pathway is involved in the plant activation of aromatic amines.

UR - http://www.scopus.com/inward/record.url?scp=0026386282&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026386282&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:0026386282

SN - 0803114222

SP - 287

EP - 296

BT - ASTM Special Technical Publication

A2 - Gorsuch, Joseph W.

A2 - Lower, William R.

A2 - Lewis, Michael A.

A2 - Sandhu, Shahbeg

A2 - Wang, Wuncheng

PB - Publ by ASTM

ER -