The scanning laser acoustic microscope (SLAM) at 100 MHz and backscattering acoustic technique (BAT) at 10–40 MHz were used to examine normal canine skin. Skin specimens from four animals and from four locations on the animal were analyzed biochemically and morphologically as well as acoustically. At 100 MHz, the mean ultrasonic speed obtained with the SLAM was 1632 ± 34 m/s and the mean attenuation coefficient was 66 ± 12 Np/cm. Using BAT, the mean integrated attenuation coefficient at 25 MHz was 13 ± 4 Np/cm. While the speed values fall within the range of values previously reported for skin from 1 to 10 MHz, the values for the attenuation coefficient using either SLAM or BAT are considerably higher than would be predicted from literature values at 1–10 MHz. Thus, the frequency dependence of the attenuation coefficient is a stronger function of frequency than the data at lower frequencies would suggest. The biochemical analyses yielded a collagen concentration of 20 ± 2 percent of the wet weight or 65 ± 12 percent of the dried defatted weight and a water concentration of 60 ± 3 percent of the wet tissue. Specimens appeared normal morphologically and sizes for structures in the skin were estimated. An analysis of variance of the SLAM data and the biochemical data showed significant animal-to-animal differences and some differences due to location on the animal.
ASJC Scopus subject areas
- Biomedical Engineering