Binding and two-dimensional crystallization of streptavidin at the air/water interface via engineered Cu-IDA chelator lipids

Kevin M. Maloney, William R. Schief, Daniel W. Pack, Wolfgang Frey, Frances H. Arnold, Viola Vogel

Research output: Contribution to journalArticlepeer-review

Abstract

Metal-chelating iminodiacetate (IDA) lipid monolayers at the air/water interface have been engineered to promote the 2D crystallization of proteins which display solvent-accessible histidines. The physical properties of IDA lipid monolayers have been specifically tailored to allow the binding and 2D assembly of added proteins. Fluorescently labeled IDA lipid monolayers allow us to monitor the formation of the protein crystals optically. Here we review our efforts in the interfacial crystallization of streptavidin as monitored using fluorescence, Brewster angle and transmission electron microscopy and discuss future challenges in this area.

Original languageEnglish (US)
Pages (from-to)3-18
Number of pages16
JournalCoordination Chemistry Reviews
Volume183
Issue number1
DOIs
StatePublished - Mar 1999

Keywords

  • Air/water interface
  • Brewster angle microscopy
  • Cu(II)
  • Fluorescence microscopy
  • Iminodiacetate
  • Metal-chelating lipids
  • Monolayers

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Inorganic Chemistry
  • Materials Chemistry

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