Bile acid sodium symporter BASS6 can transport glycolate and is involved in photorespiratory metabolism in Arabidopsis thaliana

Paul F. South, Berkley J. Walker, Amanda P. Cavanagh, Vivien Rolland, Murray Badger, Donald R. Ort

Research output: Contribution to journalArticle

Abstract

Photorespiration is an energy-intensive process that recycles 2-phosphoglycolate, a toxic product of the Rubisco oxygenation reaction. The photorespiratory pathway is highly compartmentalized, involving the chloroplast, peroxisome, cytosol, and mitochondria. Though the soluble enzymes involved in photorespiration are well characterized, very few membrane transporters involved in photorespiration have been identified to date. In this work, Arabidopsis thaliana plants containing a T-DNA disruption of the bile acid sodium symporter BASS6 show decreased photosynthesis and slower growth under ambient, but not elevated CO2. Exogenous expression of BASS6 complemented this photorespiration mutant phenotype. In addition, metabolite analysis and genetic complementation of glycolate transport in yeast showed that BASS6 was capable of glycolate transport. This is consistent with its involvement in the photorespiratory export of glycolate from Arabidopsis chloroplasts. An Arabidopsis double knockout line of both BASS6 and the glycolate/glycerate transporter PLGG1 (bass6, plgg1) showed an additive growth defect, an increase in glycolate accumulation, and reductions in photosynthetic rates compared with either single mutant. Our data indicate that BASS6 and PLGG1 partner in glycolate export from the chloroplast, whereas PLGG1 alone accounts for the import of glycerate. BASS6 and PLGG1 therefore balance the export of two glycolate molecules with the import of one glycerate molecule during photorespiration.

Original languageEnglish (US)
Pages (from-to)808-823
Number of pages16
JournalPlant Cell
Volume29
Issue number4
DOIs
StatePublished - Apr 2017

Fingerprint

glycolic acid
symporters
Symporters
photorespiration
bile acids
Bile Acids and Salts
Arabidopsis
Arabidopsis thaliana
Sodium
sodium
metabolism
chloroplasts
Chloroplasts
imports
transporters
genetic complementation
mutants
peroxisomes
ribulose-bisphosphate carboxylase
Ribulose-Bisphosphate Carboxylase

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Bile acid sodium symporter BASS6 can transport glycolate and is involved in photorespiratory metabolism in Arabidopsis thaliana. / South, Paul F.; Walker, Berkley J.; Cavanagh, Amanda P.; Rolland, Vivien; Badger, Murray; Ort, Donald R.

In: Plant Cell, Vol. 29, No. 4, 04.2017, p. 808-823.

Research output: Contribution to journalArticle

South, Paul F. ; Walker, Berkley J. ; Cavanagh, Amanda P. ; Rolland, Vivien ; Badger, Murray ; Ort, Donald R. / Bile acid sodium symporter BASS6 can transport glycolate and is involved in photorespiratory metabolism in Arabidopsis thaliana. In: Plant Cell. 2017 ; Vol. 29, No. 4. pp. 808-823.
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