Biaxial Dielectrophoresis Force Spectroscopy: A Stoichiometric Approach for Examining Intermolecular Weak Binding Interactions

In Soo Park, Tae Joon Kwak, Gyudo Lee, Myeonggu Son, Jeong Woo Choi, Seungyeop Choi, Kihwan Nam, Sei Young Lee, Woo Jin Chang, Kilho Eom, Dae Sung Yoon, Sangyoup Lee, Rashid Bashir, Sang Woo Lee

Research output: Contribution to journalArticle

Abstract

The direct quantification of weak intermolecular binding interactions is very important for many applications in biology and medicine. Techniques that can be used to investigate such interactions under a controlled environment, while varying different parameters such as loading rate, pulling direction, rupture event measurements, and the use of different functionalized probes, are still lacking. Herein, we demonstrate a biaxial dielectrophoresis force spectroscopy (BDFS) method that can be used to investigate weak unbinding events in a high-throughput manner under controlled environments and by varying the pulling direction (i.e., transverse and/or vertical axes) as well as the loading rate. With the BDFS system, we can quantitatively analyze binding interactions related to hydrogen bonding or ionic attractions between functionalized microbeads and a surface within a microfluidic device. Our BDFS system allowed for the characterization of the number of bonds involved in an interaction, bond affinity, kinetic rates, and energy barrier heights and widths from different regimes of the energy landscape.

Original languageEnglish (US)
Pages (from-to)4011-4019
Number of pages9
JournalACS Nano
Volume10
Issue number4
DOIs
StatePublished - Apr 26 2016

Fingerprint

Electrophoresis
Spectroscopy
loading rate
pulling
spectroscopy
Energy barriers
interactions
Microfluidics
Medicine
microfluidic devices
Hydrogen bonds
biology
medicine
Throughput
attraction
affinity
Kinetics
energy
probes
kinetics

Keywords

  • dielectrophoresis force spectroscopy
  • energy landscape
  • intermolecular weak binding interactions
  • microfluidic device
  • pulling direction

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Biaxial Dielectrophoresis Force Spectroscopy : A Stoichiometric Approach for Examining Intermolecular Weak Binding Interactions. / Park, In Soo; Kwak, Tae Joon; Lee, Gyudo; Son, Myeonggu; Choi, Jeong Woo; Choi, Seungyeop; Nam, Kihwan; Lee, Sei Young; Chang, Woo Jin; Eom, Kilho; Yoon, Dae Sung; Lee, Sangyoup; Bashir, Rashid; Lee, Sang Woo.

In: ACS Nano, Vol. 10, No. 4, 26.04.2016, p. 4011-4019.

Research output: Contribution to journalArticle

Park, IS, Kwak, TJ, Lee, G, Son, M, Choi, JW, Choi, S, Nam, K, Lee, SY, Chang, WJ, Eom, K, Yoon, DS, Lee, S, Bashir, R & Lee, SW 2016, 'Biaxial Dielectrophoresis Force Spectroscopy: A Stoichiometric Approach for Examining Intermolecular Weak Binding Interactions', ACS Nano, vol. 10, no. 4, pp. 4011-4019. https://doi.org/10.1021/acsnano.5b05286
Park, In Soo ; Kwak, Tae Joon ; Lee, Gyudo ; Son, Myeonggu ; Choi, Jeong Woo ; Choi, Seungyeop ; Nam, Kihwan ; Lee, Sei Young ; Chang, Woo Jin ; Eom, Kilho ; Yoon, Dae Sung ; Lee, Sangyoup ; Bashir, Rashid ; Lee, Sang Woo. / Biaxial Dielectrophoresis Force Spectroscopy : A Stoichiometric Approach for Examining Intermolecular Weak Binding Interactions. In: ACS Nano. 2016 ; Vol. 10, No. 4. pp. 4011-4019.
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