TY - JOUR
T1 - Beyond the sea
T2 - Crepidula atrasolea as a spiralian model system
AU - Henry, Jonathan Q.
AU - Lesoway, Maryna P.
AU - Perry, Kimberly J.
AU - Osborne, Chen Cornelia
AU - Shankland, Marty
AU - Lyons, Deirdre C.
N1 - Funding Information:
Acknowledgements The authors thank Dr. Rachel Collin for suggesting that C. atrasolea would represent a convenient model system. We also thank Sherry Reed, Seabird McKeon, and other members of the Smithsonian Marine Station in Fort Pierce, FL for their assistance in collecting these animals. In addition, we thank Drs. Leslie Babonis and Mark Martindale of the Whitney Marine lab in St Augustine, FL for their assistance. We are grateful for the support provided by Dr. Alvaro Hernandez, Director of the High-Throughput Sequencing and Genotyping Unit at the Roy J. Carver Biotechnology Center, University of Illinois. The authors acknowledge the invaluable support of the National Science Foundation (NSF). JQH (JJH), KJP, MPL, CCO were supported by NSF grant IOS-1558061. MPL was supported by a Postdoctoral Fellowship from the Fonds de recherche du Québec - Nature et technologies (FRQ-NT). MPL thanks the Society for Developmental Biology for an Emerging Models Grant in support of work on C. atrasolea. DCL thanks Dr. David R. McClay for his support.
Publisher Copyright:
© 2017 UPV/EHU Press.
PY - 2017
Y1 - 2017
N2 - This paper introduces the black-footed slipper snail, Crepidula atrasolea, as a new model for biological studies in the Spiralia. C. atrasolea is a calyptraeid gastropod, and congener of the Atlantic slipper snail, C. fornicata. Like C. fornicata, C. atrasolea shares a sedentary, filter-feeding, protandrous lifestyle, but is preferable as a developmental model because of its short generation time, year-round reproduction, and direct development. In our lab, individuals go from egg to reproductive females in under six months, as compared to an estimated 1-2 years for C. fornicata. Here we provide details for collecting and transporting animals, setting up inland aquaria, and maintaining laboratory colonies of C. atrasolea. We also describe early development, which is similar to that in other calyptraeids. Females brood encapsulated embryos for three weeks, which hatch as “crawl-away” juveniles. We also present a developmental transcriptome for C. atrasolea, covering early cleavage through late organogenesis stages, as a useful tool for future studies of gene expression and function. We provide this information to the broader developmental community to facilitate widespread use of this system.
AB - This paper introduces the black-footed slipper snail, Crepidula atrasolea, as a new model for biological studies in the Spiralia. C. atrasolea is a calyptraeid gastropod, and congener of the Atlantic slipper snail, C. fornicata. Like C. fornicata, C. atrasolea shares a sedentary, filter-feeding, protandrous lifestyle, but is preferable as a developmental model because of its short generation time, year-round reproduction, and direct development. In our lab, individuals go from egg to reproductive females in under six months, as compared to an estimated 1-2 years for C. fornicata. Here we provide details for collecting and transporting animals, setting up inland aquaria, and maintaining laboratory colonies of C. atrasolea. We also describe early development, which is similar to that in other calyptraeids. Females brood encapsulated embryos for three weeks, which hatch as “crawl-away” juveniles. We also present a developmental transcriptome for C. atrasolea, covering early cleavage through late organogenesis stages, as a useful tool for future studies of gene expression and function. We provide this information to the broader developmental community to facilitate widespread use of this system.
KW - Aquaculture
KW - Development
KW - Gastropoda
KW - Mollusca
KW - Spiralia
KW - Transcriptome
UR - http://www.scopus.com/inward/record.url?scp=85033804396&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85033804396&partnerID=8YFLogxK
U2 - 10.1387/ijdb.170110jh
DO - 10.1387/ijdb.170110jh
M3 - Article
C2 - 29139534
AN - SCOPUS:85033804396
SN - 0214-6282
VL - 61
SP - 479
EP - 493
JO - International Journal of Developmental Biology
JF - International Journal of Developmental Biology
IS - 8-9
ER -