Beyond the sea: Crepidula atrasolea as a spiralian model system

Jonathan Joseph Henry, Maryna P. Lesoway, Kimberly J. Perry, Chen Cornelia Osborne, Marty Shankland, Deirdre C. Lyons

Research output: Contribution to journalArticle

Abstract

This paper introduces the black-footed slipper snail, Crepidula atrasolea, as a new model for biological studies in the Spiralia. C. atrasolea is a calyptraeid gastropod, and congener of the Atlantic slipper snail, C. fornicata. Like C. fornicata, C. atrasolea shares a sedentary, filter-feeding, protandrous lifestyle, but is preferable as a developmental model because of its short generation time, year-round reproduction, and direct development. In our lab, individuals go from egg to reproductive females in under six months, as compared to an estimated 1-2 years for C. fornicata. Here we provide details for collecting and transporting animals, setting up inland aquaria, and maintaining laboratory colonies of C. atrasolea. We also describe early development, which is similar to that in other calyptraeids. Females brood encapsulated embryos for three weeks, which hatch as “crawl-away” juveniles. We also present a developmental transcriptome for C. atrasolea, covering early cleavage through late organogenesis stages, as a useful tool for future studies of gene expression and function. We provide this information to the broader developmental community to facilitate widespread use of this system.

Original languageEnglish (US)
Pages (from-to)479-493
Number of pages15
JournalInternational Journal of Developmental Biology
Volume61
Issue number8-9
DOIs
StatePublished - Jan 1 2017

Fingerprint

Snails
Oceans and Seas
Biological Models
Gastropoda
Organogenesis
Transcriptome
Reproduction
Ovum
Life Style
Embryonic Structures
Gene Expression

Keywords

  • Aquaculture
  • Development
  • Gastropoda
  • Mollusca
  • Spiralia
  • Transcriptome

ASJC Scopus subject areas

  • Embryology
  • Developmental Biology

Cite this

Henry, J. J., Lesoway, M. P., Perry, K. J., Osborne, C. C., Shankland, M., & Lyons, D. C. (2017). Beyond the sea: Crepidula atrasolea as a spiralian model system. International Journal of Developmental Biology, 61(8-9), 479-493. https://doi.org/10.1387/ijdb.170110jh

Beyond the sea : Crepidula atrasolea as a spiralian model system. / Henry, Jonathan Joseph; Lesoway, Maryna P.; Perry, Kimberly J.; Osborne, Chen Cornelia; Shankland, Marty; Lyons, Deirdre C.

In: International Journal of Developmental Biology, Vol. 61, No. 8-9, 01.01.2017, p. 479-493.

Research output: Contribution to journalArticle

Henry, JJ, Lesoway, MP, Perry, KJ, Osborne, CC, Shankland, M & Lyons, DC 2017, 'Beyond the sea: Crepidula atrasolea as a spiralian model system', International Journal of Developmental Biology, vol. 61, no. 8-9, pp. 479-493. https://doi.org/10.1387/ijdb.170110jh
Henry, Jonathan Joseph ; Lesoway, Maryna P. ; Perry, Kimberly J. ; Osborne, Chen Cornelia ; Shankland, Marty ; Lyons, Deirdre C. / Beyond the sea : Crepidula atrasolea as a spiralian model system. In: International Journal of Developmental Biology. 2017 ; Vol. 61, No. 8-9. pp. 479-493.
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