Betaine-homocysteine methyltransferase expression in porcine and human tissues and chromosomal localization of the human gene

Sara L.F. Sunden, Murty S. Renduchintala, Eric I. Park, Steven D. Miklasz, Timothy A. Garrow

Research output: Contribution to journalArticlepeer-review

Abstract

We have prepared antibodies against porcine liver betaine-homocysteine methyltransferase (BHMT; EC 2.1.1.5) and recently cloned cDNAs encoding the porcine and human liver enzymes. Porcine tissues were evaluated for BHMT expression by measuring catalytic activity and Western analysis. Liver and kidney were the only organs tested that had immunodetectable levels of BHMT, and these organs expressed high levels of enzyme activity. BHMT was expressed in the kidney cortex and not the medulla. Porcine pancreas, brain, heart, lung, and spleen were devoid of BHMT activity and immunodetectable protein. Human tissues were tested for BHMT expression by Northern analysis. Human liver and kidney were the only organs tested that expressed BHMT mRNA. Human pancreas, brain, heart, skeletal muscle, spleen, and placenta were devoid of BHMT mRNA. The human BHMT gene has been mapped to chromosome 5q13.1-q15.

Original languageEnglish (US)
Pages (from-to)171-174
Number of pages4
JournalArchives of Biochemistry and Biophysics
Volume345
Issue number1
DOIs
StatePublished - Sep 1 1997

Keywords

  • Betaine
  • Betaine-homocysteine methyltransferase
  • Homocysteine
  • Human chromosome 5
  • Methionine

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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