The objectives of this study were to evaluate the effect on markers of inflammation in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages of protein hydrolysates of the common bean (Phaseolus vulgaris L.) varieties Negro 8025 and Pinto Durango. Cell viability was determined and the percentage of viable cells was calculated and concentrations that allowed >80% cell viability were used to determine the effect on markers of inflammation. Alcalase hydrolysates of common beans inhibited cyclooxygenase-2 expression, prostaglandin E2 production, inducible nitric oxide synthase expression and nitric oxide production, for Pinto Durango, with IC50 values of 34.9 ± 0.3, 13.9 ± 0.3, 5.0 ± 0.1 and 3.7 ± 0.2 μM, respectively while var. Negro needed 43.6 ± 0.2, 61.3 ± 0.3, 14.2 ± 0.3 and 48.2 ± 0.1 μM. In conclusion, hydrolysates from common beans can be used to combat inflammatory associated diseases.