Baseline sensitivity of Ascochyta rabiei to azoxystrobin, pyraclostrobin, and boscalid

K. A. Wise, C. A. Bradley, J. S. Pasche, N. C. Gudmestad, F. M. Dugan, W. Chen

Research output: Contribution to journalArticle

Abstract

Ascochyta rabiei, causal agent of Ascochyta blight on chickpea (Cicer arietinum), can cause severe yield loss in the United States. Growers rely on applications of fungicides with site-specific modes of action such as the quinone outside inhibiting (QoI) fungicides azoxystrobin and pyraclostrobin, and the carboximide fungicide boscalid, to manage disease. In all, 51 isolates collected prior to QoI fungicide registration and 71 isolates collected prior to boscalid registration in the United States were tested in an in vitro assay to determine the effective fungicide concentration at which 50% of conidial germination was inhibited (EC50) for each isolate-fungicide combination. The effect of salicylhydroxamic acid (SHAM) on conidia of A. rabiei in the presence and absence of azoxystrobin also was assessed to determine whether the fungus is capable of using alternative respiration. Five of nine A. rabiei isolates tested had significantly higher (P ≤ 0.05) EC50 values when SHAM was not included in media amended with azoxystrobin, indicating that A. rabiei has the potential to use alternative respiration to overcome fungicide toxicity in vitro. EC50 values of azoxystrobin and pyraclostrobin ranged from 0.0182 to 0.0338 μg/ml and from 0.0012 to 0.0033 pg/ml, with mean values of 0.0272 and 0.0023 μg/ml, respectively. EC 50 values of boscalid ranged from 0.0177 to 0.4960 μg/ml, with a mean of 0.1903 μg/ml. Establishment of these baselines is the first step in developing a monitoring program to determine whether shifts in sensitivity to these fungicides are occurring in the A. rabiei pathogen population.

Original languageEnglish (US)
Pages (from-to)295-300
Number of pages6
JournalPlant disease
Volume92
Issue number2
DOIs
StatePublished - Feb 1 2008

Fingerprint

boscalid
Ascochyta rabiei
pyraclostrobin
fungicides
cell respiration
quinones
Ascochyta
acids
Cicer arietinum
azoxystrobin
blight
pesticide application
conidia
growers
mechanism of action
germination
toxicity
fungi
pathogens
monitoring

Keywords

  • Didymella rabiei
  • Fungicide resistance

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Wise, K. A., Bradley, C. A., Pasche, J. S., Gudmestad, N. C., Dugan, F. M., & Chen, W. (2008). Baseline sensitivity of Ascochyta rabiei to azoxystrobin, pyraclostrobin, and boscalid. Plant disease, 92(2), 295-300. https://doi.org/10.1094/PDIS-92-2-0295

Baseline sensitivity of Ascochyta rabiei to azoxystrobin, pyraclostrobin, and boscalid. / Wise, K. A.; Bradley, C. A.; Pasche, J. S.; Gudmestad, N. C.; Dugan, F. M.; Chen, W.

In: Plant disease, Vol. 92, No. 2, 01.02.2008, p. 295-300.

Research output: Contribution to journalArticle

Wise, KA, Bradley, CA, Pasche, JS, Gudmestad, NC, Dugan, FM & Chen, W 2008, 'Baseline sensitivity of Ascochyta rabiei to azoxystrobin, pyraclostrobin, and boscalid', Plant disease, vol. 92, no. 2, pp. 295-300. https://doi.org/10.1094/PDIS-92-2-0295
Wise KA, Bradley CA, Pasche JS, Gudmestad NC, Dugan FM, Chen W. Baseline sensitivity of Ascochyta rabiei to azoxystrobin, pyraclostrobin, and boscalid. Plant disease. 2008 Feb 1;92(2):295-300. https://doi.org/10.1094/PDIS-92-2-0295
Wise, K. A. ; Bradley, C. A. ; Pasche, J. S. ; Gudmestad, N. C. ; Dugan, F. M. ; Chen, W. / Baseline sensitivity of Ascochyta rabiei to azoxystrobin, pyraclostrobin, and boscalid. In: Plant disease. 2008 ; Vol. 92, No. 2. pp. 295-300.
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