Base-induced delignification of miscanthus x giganteus studied by three-dimensional confocal raman imaging

Li Qiang Chu, Rachel Masyuko, Jonathan V. Sweedler, Paul W. Bohn

Research output: Contribution to journalArticle

Abstract

Confocal raman microscopy has been used to monitor the structural and chemical changes upon NaOH treatment of Miscanthus x giganteus, a potential energy crop and a model lignocellulosic material. Longitudinal and transversal-section images of the parenchyma cells in raw miscanthus samples reveal that lignin and cellulose are collocated in the cell wall and that a globular structure, composed predominantly of hemicellulose and lignin is associated with the interior cell wall. NaOH treatment results in the complete removal of lignin at long processing time but leaves the cellulose largely undisturbed as evidenced by the lack of conversion from type I to type II cellulose. Depth profiling images of partially processed (short exposure time) parenchyma cells reveal that lignin is removed preferentially from the interior surface of the cell wall as indicated by the anisotropic distribution of lignin and cellulose across the cell wall in partially processed samples. These spatially resolved chemical changes are important, because they illustrate how even simple pre-processing protocols can develop complex molecular profiles by differential rates of attack on the major components of the cell wall.

Original languageEnglish (US)
Pages (from-to)4919-4925
Number of pages7
JournalBioresource Technology
Volume101
Issue number13
DOIs
StatePublished - Jul 1 2010

Fingerprint

Delignification
Lignin
lignin
Cellulose
Cells
cellulose
Imaging techniques
energy crop
Depth profiling
Processing
Potential energy
potential energy
Crops
microscopy
Microscopic examination

Keywords

  • Confocal raman microscopy
  • Delignification
  • Lignocellulosic material
  • Miscanthus x giganteus
  • NaOH treatment

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

Cite this

Base-induced delignification of miscanthus x giganteus studied by three-dimensional confocal raman imaging. / Chu, Li Qiang; Masyuko, Rachel; Sweedler, Jonathan V.; Bohn, Paul W.

In: Bioresource Technology, Vol. 101, No. 13, 01.07.2010, p. 4919-4925.

Research output: Contribution to journalArticle

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