Abstract
Imidazole glycerol phosphate synthase (HisF) has been proposed to be the product of duplication of a gene encoding a (β/α)4-half barrel followed by fusion to encode the compmete (β/α)8-barrel. In support of this evolutionary scenario, the N- and C-terminal (β/α)4-half barrels of TrpF have been separately expressed and purified. Each assumes a stable, soluble homodimeric structure, but neither is catalytically active; when expressed together, a functional heterodimer is formed.
Original language | English (US) |
---|---|
Pages (from-to) | 5-7 |
Number of pages | 3 |
Journal | Nature Structural Biology |
Volume | 8 |
Issue number | 1 |
DOIs | |
State | Published - Jan 19 2001 |
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ASJC Scopus subject areas
- Structural Biology
- Biochemistry
- Genetics
Cite this
Barrels in pieces? / Gerlt, John A.; Babbitt, Patricia C.
In: Nature Structural Biology, Vol. 8, No. 1, 19.01.2001, p. 5-7.Research output: Contribution to journal › Short survey
}
TY - JOUR
T1 - Barrels in pieces?
AU - Gerlt, John A.
AU - Babbitt, Patricia C.
PY - 2001/1/19
Y1 - 2001/1/19
N2 - Imidazole glycerol phosphate synthase (HisF) has been proposed to be the product of duplication of a gene encoding a (β/α)4-half barrel followed by fusion to encode the compmete (β/α)8-barrel. In support of this evolutionary scenario, the N- and C-terminal (β/α)4-half barrels of TrpF have been separately expressed and purified. Each assumes a stable, soluble homodimeric structure, but neither is catalytically active; when expressed together, a functional heterodimer is formed.
AB - Imidazole glycerol phosphate synthase (HisF) has been proposed to be the product of duplication of a gene encoding a (β/α)4-half barrel followed by fusion to encode the compmete (β/α)8-barrel. In support of this evolutionary scenario, the N- and C-terminal (β/α)4-half barrels of TrpF have been separately expressed and purified. Each assumes a stable, soluble homodimeric structure, but neither is catalytically active; when expressed together, a functional heterodimer is formed.
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UR - http://www.scopus.com/inward/citedby.url?scp=0035174241&partnerID=8YFLogxK
U2 - 10.1038/83048
DO - 10.1038/83048
M3 - Short survey
C2 - 11135656
AN - SCOPUS:0035174241
VL - 8
SP - 5
EP - 7
JO - Nature Structural and Molecular Biology
JF - Nature Structural and Molecular Biology
SN - 1545-9993
IS - 1
ER -