Barrels in pieces?

John A. Gerlt; Patricia C. Babbitt

doi:10.1038/83048

Barrels in pieces?

John A. Gerlt, Patricia C. Babbitt

Research output: Contribution to journal › Short survey › peer-review

Abstract

Imidazole glycerol phosphate synthase (HisF) has been proposed to be the product of duplication of a gene encoding a (β/α)₄-half barrel followed by fusion to encode the compmete (β/α)₈-barrel. In support of this evolutionary scenario, the N- and C-terminal (β/α)₄-half barrels of TrpF have been separately expressed and purified. Each assumes a stable, soluble homodimeric structure, but neither is catalytically active; when expressed together, a functional heterodimer is formed.

Original language	English (US)
Pages (from-to)	5-7
Number of pages	3
Journal	Nature Structural Biology
Volume	8
Issue number	1
DOIs	https://doi.org/10.1038/83048
State	Published - Jan 19 2001

ASJC Scopus subject areas

Structural Biology
Biochemistry
Genetics

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@article{301ed451157e4704bf3c1e8e38c474ae,

title = "Barrels in pieces?",

abstract = "Imidazole glycerol phosphate synthase (HisF) has been proposed to be the product of duplication of a gene encoding a (β/α)4-half barrel followed by fusion to encode the compmete (β/α)8-barrel. In support of this evolutionary scenario, the N- and C-terminal (β/α)4-half barrels of TrpF have been separately expressed and purified. Each assumes a stable, soluble homodimeric structure, but neither is catalytically active; when expressed together, a functional heterodimer is formed.",

author = "Gerlt, {John A.} and Babbitt, {Patricia C.}",

year = "2001",

month = jan,

day = "19",

doi = "10.1038/83048",

language = "English (US)",

volume = "8",

pages = "5--7",

journal = "Nature Structural and Molecular Biology",

issn = "1545-9993",