Barrels in pieces?

John Alan Gerlt, Patricia C. Babbitt

Research output: Contribution to journalShort survey

Abstract

Imidazole glycerol phosphate synthase (HisF) has been proposed to be the product of duplication of a gene encoding a (β/α)4-half barrel followed by fusion to encode the compmete (β/α)8-barrel. In support of this evolutionary scenario, the N- and C-terminal (β/α)4-half barrels of TrpF have been separately expressed and purified. Each assumes a stable, soluble homodimeric structure, but neither is catalytically active; when expressed together, a functional heterodimer is formed.

Original languageEnglish (US)
Pages (from-to)5-7
Number of pages3
JournalNature Structural Biology
Volume8
Issue number1
DOIs
StatePublished - Jan 19 2001

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Gene encoding
Gene Duplication
Fusion reactions
imidazole glycerol phosphate synthase

ASJC Scopus subject areas

  • Biochemistry
  • Structural Biology
  • Genetics

Cite this

Barrels in pieces? / Gerlt, John Alan; Babbitt, Patricia C.

In: Nature Structural Biology, Vol. 8, No. 1, 19.01.2001, p. 5-7.

Research output: Contribution to journalShort survey

Gerlt, John Alan ; Babbitt, Patricia C. / Barrels in pieces?. In: Nature Structural Biology. 2001 ; Vol. 8, No. 1. pp. 5-7.
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