Barrels in pieces?

John A. Gerlt, Patricia C. Babbitt

Research output: Contribution to journalShort surveypeer-review


Imidazole glycerol phosphate synthase (HisF) has been proposed to be the product of duplication of a gene encoding a (β/α)4-half barrel followed by fusion to encode the compmete (β/α)8-barrel. In support of this evolutionary scenario, the N- and C-terminal (β/α)4-half barrels of TrpF have been separately expressed and purified. Each assumes a stable, soluble homodimeric structure, but neither is catalytically active; when expressed together, a functional heterodimer is formed.

Original languageEnglish (US)
Pages (from-to)5-7
Number of pages3
JournalNature Structural Biology
Issue number1
StatePublished - Jan 19 2001

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Genetics


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