Balancing selection contributed to domestication of autopolyploid sugarcane (Saccharum officinarum L.)

Jie Arro, Jong Won Park, Ching Man Wai, Robert VanBuren, Yong Bao Pan, Chifumi Nagai, Jorge da Silva, Ray R Ming

Research output: Contribution to journalArticle

Abstract

Sugarcane was domesticated in New Guinea about 10,000 years ago, and the domesticated species Saccharum officinarum is autooctoploid. For diploid domesticated crops, genetic diversity is reduced for those genes controlling favorable traits. However, the domestication traits in sugarcane such as sugar content and biomass yield are controlled by multiple genes with multiple alleles. A genomics approach to identify genes involved in the transition from wild to domesticated may provide useful insight into complex polyploid traits such as sucrose accumulation. Fifteen accessions each of domesticated S. officinarum and the wild species S. robustum and eighteen accessions of S. spontaneum were used for sequencing of leaf and stalk transcriptomes. We found high allelic diversity among genes expressed in the stalk tissues where the domesticated S. officinarum (Fis = 0.69) surprisingly has higher allele diversity than its wild relative S. spontaneum (Fis = 0.41). However, there were no SNP loci with extremely high FST values despite the observed higher average FST among the three species, indicative of the action of balancing selection. This is corroborated by nucleotide diversity and site frequency spectrum (SFS) patterns that show that majority of expressed genes in S. officinarum have comparable per-site heterozygosity to the wild species. These candidate domestication genes, bearing signatures of balancing selection and excess singleton SNPs in S. officinarum, perturbs pathways involved in sucrose and starch metabolism.

Original languageEnglish (US)
Pages (from-to)477-493
Number of pages17
JournalEuphytica
Volume209
Issue number2
DOIs
StatePublished - May 1 2016

Fingerprint

autopolyploidy
Saccharum
Saccharum officinarum
domestication
sugarcane
genes
Genes
Single Nucleotide Polymorphism
Sucrose
sucrose
alleles
Alleles
New Guinea
wild relatives
Polyploidy
transcriptome
polyploidy
sugar content
Genomics
Domestication

Keywords

  • Domestication
  • Polyploid
  • Population genetics
  • Selection scan
  • Sugarcane

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science
  • Horticulture

Cite this

Balancing selection contributed to domestication of autopolyploid sugarcane (Saccharum officinarum L.). / Arro, Jie; Park, Jong Won; Wai, Ching Man; VanBuren, Robert; Pan, Yong Bao; Nagai, Chifumi; da Silva, Jorge; Ming, Ray R.

In: Euphytica, Vol. 209, No. 2, 01.05.2016, p. 477-493.

Research output: Contribution to journalArticle

Arro, J, Park, JW, Wai, CM, VanBuren, R, Pan, YB, Nagai, C, da Silva, J & Ming, RR 2016, 'Balancing selection contributed to domestication of autopolyploid sugarcane (Saccharum officinarum L.)', Euphytica, vol. 209, no. 2, pp. 477-493. https://doi.org/10.1007/s10681-016-1672-8
Arro, Jie ; Park, Jong Won ; Wai, Ching Man ; VanBuren, Robert ; Pan, Yong Bao ; Nagai, Chifumi ; da Silva, Jorge ; Ming, Ray R. / Balancing selection contributed to domestication of autopolyploid sugarcane (Saccharum officinarum L.). In: Euphytica. 2016 ; Vol. 209, No. 2. pp. 477-493.
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