Bacterial Genome Containing Chimeric DNA-RNA Sequences

Angad P. Mehta, Yiyang Wang, Sean A. Reed, Lubica Supekova, Tsotne Javahishvili, John C. Chaput, Peter G. Schultz

Research output: Contribution to journalArticlepeer-review


Almost five decades ago Crick, Orgel, and others proposed the RNA world hypothesis. Subsequent studies have raised the possibility that RNA might be able to support both genotype and phenotype, and the function of RNA templates has been studied in terms of evolution, replication, and catalysis. Recently, we engineered strains of E. coli in which a large fraction of 2′-deoxycytidine in the genome is substituted with the modified base 5-hydroxymethyl-2′-deoxycytidine. We now report the generation of mutant strains derived from these engineered bacteria that show significant (∼40-50%) ribonucleotide content in their genome. We have begun to characterize the properties of these chimeric genomes and the corresponding strains to determine the circumstances under which E. coli can incorporate ribonucleotides into its genome and herein report our initial observations.

Original languageEnglish (US)
Pages (from-to)11464-11473
Number of pages10
JournalJournal of the American Chemical Society
Issue number36
StatePublished - Sep 12 2018
Externally publishedYes

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry


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