TY - JOUR
T1 - Bacteria-mediated delivery of nanoparticles and cargo into cells
AU - Akin, Demir
AU - Sturgis, Jennifer
AU - Ragheb, Kathy
AU - Sherman, Debby
AU - Burkholder, Kristin
AU - Robinson, J. Paul
AU - Bhunia, Arun K.
AU - Mohammed, Sulma
AU - Bashir, Rashid
N1 - Funding Information:
The authors would like to thank C. Koons, Drug Discovery Shared Resource of Purdue Cancer Center, for her help with the in vivo studies, S. Leavesly for his inputs in the initial bioluminescence imaging studies, C. Buck for assisting in the use of the facilities at Bindley Biosciences Center, and the Weldon School of Biomedical Engineering for funding the work. D.A. was supported by funds from NIH NIBIB. Correspondence and requests for materials should be addressed to D.A. and R.B. Supplementary information accompanies this paper on www.nature.com/naturenanotechnology.
PY - 2007/7
Y1 - 2007/7
N2 - Nanoparticles and bacteria can be used, independently, to deliver genes and proteins into mammalian cells for monitoring or altering gene expression and protein production. Here, we show the simultaneous use of nanoparticles and bacteria to deliver DNA-based model drug molecules in vivo and in vitro. In our approach, cargo (in this case, a fluorescent or a bioluminescent gene) is loaded onto the nanoparticles, which are carried on the bacteria surface. When incubated with cells, the cargo-carrying bacteria ('microbots') were internalized by the cells, and the genes released from the nanoparticles were expressed in the cells. Mice injected with microbots also successfully expressed the genes as seen by the luminescence in different organs. This new approach may be used to deliver different types of cargo into live animals and a variety of cells in culture without the need for complicated genetic manipulations.
AB - Nanoparticles and bacteria can be used, independently, to deliver genes and proteins into mammalian cells for monitoring or altering gene expression and protein production. Here, we show the simultaneous use of nanoparticles and bacteria to deliver DNA-based model drug molecules in vivo and in vitro. In our approach, cargo (in this case, a fluorescent or a bioluminescent gene) is loaded onto the nanoparticles, which are carried on the bacteria surface. When incubated with cells, the cargo-carrying bacteria ('microbots') were internalized by the cells, and the genes released from the nanoparticles were expressed in the cells. Mice injected with microbots also successfully expressed the genes as seen by the luminescence in different organs. This new approach may be used to deliver different types of cargo into live animals and a variety of cells in culture without the need for complicated genetic manipulations.
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U2 - 10.1038/nnano.2007.149
DO - 10.1038/nnano.2007.149
M3 - Article
C2 - 18654330
AN - SCOPUS:34447118226
SN - 1748-3387
VL - 2
SP - 441
EP - 449
JO - Nature Nanotechnology
JF - Nature Nanotechnology
IS - 7
ER -