Bacteria-Assisted Activation of Antimicrobial Polypeptides by a Random-Coil to Helix Transition

Menghua Xiong, Zhiyuan Han, Ziyuan Song, Jin Yu, Hanze Ying, Lichen Yin, Jianjun Cheng

Research output: Contribution to journalArticlepeer-review

Abstract

The application of antimicrobial peptides (AMPs) is largely hindered by their non-specific toxicity against mammalian cells, which is usually associated with helical structure, hydrophobicity, and charge density. A random coil-to-helix transition mechanism has now been introduced into the design of AMPs, minimizing the toxicity against mammalian cells while maintaining high antimicrobial activity. By incorporating anionic phosphorylated tyrosine into the cationic polypeptide, the helical structure of AMPs was distorted owing to the side-chain charge interaction. Together with the decreased charge density, the AMPs exhibited inhibited toxicity against mammalian cells. At the infectious site, the AMPs can be activated by bacterial phosphatase to restore the helical structure, thus contributing to strong membrane disruptive capability and potent antimicrobial activity. This bacteria-activated system is an effective strategy to enhance the therapeutic selectivity of AMPs.

Original languageEnglish (US)
Pages (from-to)10826-10829
Number of pages4
JournalAngewandte Chemie - International Edition
Volume56
Issue number36
DOIs
StatePublished - Aug 28 2017

Keywords

  • antimicrobial activity
  • bacteria
  • helical structures
  • peptides
  • phosphatase

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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