Backbone resonance assignments of the C2 domain of coagulation factor VIII

Kristin M. Nuzzio; David B. Cullinan; Valerie A. Novakovic; John M. Boettcher; Chad M. Rienstra; Gary E. Gilbert; James D. Baleja

doi:10.1007/s12104-012-9370-5

Backbone resonance assignments of the C2 domain of coagulation factor VIII

Kristin M. Nuzzio, David B. Cullinan, Valerie A. Novakovic, John M. Boettcher, Chad M. Rienstra, Gary E. Gilbert, James D. Baleja

Research output: Contribution to journal › Article › peer-review

Abstract

Factor VIII (FVIII, other clotting factors are named similarly) is a glycoprotein that circulates in the plasma bound to von Willebrand factor. During the blood coagulation cascade, activated FVIII (FVIIIa) binds to FIXa and activates FX in the presence of calcium ions and phospholipid membranes. The C1 and C2 domains mediate membrane binding that is essential for activation of the FVIIIa-FIXa complex. Here, ¹H, ¹³C, and ¹⁵N backbone chemical shift assignments are reported for the C2 domain of FVIII, including assignments for the residues in solvent-exposed loops. The NMR resonance assignments, along with further structural studies of membrane-bound FVIII, will advance understanding of blood-clotting protein interactions.

Original language	English (US)
Pages (from-to)	31-34
Number of pages	4
Journal	Biomolecular NMR Assignments
Volume	7
Issue number	1
DOIs	https://doi.org/10.1007/s12104-012-9370-5
State	Published - Apr 2013

Keywords

Blood coagulation
C2 discoidin-like domain
FVIII
Membrane-associated protein

ASJC Scopus subject areas

Structural Biology
Biochemistry

Online availability

10.1007/s12104-012-9370-5

Library availability

Discover UIUC Full Text

Cite this

@article{16f0c73f943a419ebe2d32c267c0ba74,

title = "Backbone resonance assignments of the C2 domain of coagulation factor VIII",

abstract = "Factor VIII (FVIII, other clotting factors are named similarly) is a glycoprotein that circulates in the plasma bound to von Willebrand factor. During the blood coagulation cascade, activated FVIII (FVIIIa) binds to FIXa and activates FX in the presence of calcium ions and phospholipid membranes. The C1 and C2 domains mediate membrane binding that is essential for activation of the FVIIIa-FIXa complex. Here, 1H, 13C, and 15N backbone chemical shift assignments are reported for the C2 domain of FVIII, including assignments for the residues in solvent-exposed loops. The NMR resonance assignments, along with further structural studies of membrane-bound FVIII, will advance understanding of blood-clotting protein interactions.",

keywords = "Blood coagulation, C2 discoidin-like domain, FVIII, Membrane-associated protein",

author = "Nuzzio, {Kristin M.} and Cullinan, {David B.} and Novakovic, {Valerie A.} and Boettcher, {John M.} and Rienstra, {Chad M.} and Gilbert, {Gary E.} and Baleja, {James D.}",

note = "Funding Information: Acknowledgments This work was supported by National Institutes of Health grant P01HL42443 (to JDB), National Institutes of Health grant R01HL103999 (to CMR), and the Research Service of the Veterans Administration (GEG). Fellowship support was provided by a National Science Foundation Graduate Research Fellowship to KMN. KMN wishes to thank Gemma Comellas and Dr. Anna E. Nesbitt for insightful discussions.",

year = "2013",

month = apr,

doi = "10.1007/s12104-012-9370-5",

language = "English (US)",

volume = "7",

pages = "31--34",

journal = "Biomolecular NMR Assignments",

issn = "1874-2718",

publisher = "Springer Science and Business Media B.V.",

number = "1",

}

TY - JOUR

T1 - Backbone resonance assignments of the C2 domain of coagulation factor VIII

AU - Nuzzio, Kristin M.

AU - Cullinan, David B.

AU - Novakovic, Valerie A.

AU - Boettcher, John M.

AU - Rienstra, Chad M.

AU - Gilbert, Gary E.

AU - Baleja, James D.

N1 - Funding Information: Acknowledgments This work was supported by National Institutes of Health grant P01HL42443 (to JDB), National Institutes of Health grant R01HL103999 (to CMR), and the Research Service of the Veterans Administration (GEG). Fellowship support was provided by a National Science Foundation Graduate Research Fellowship to KMN. KMN wishes to thank Gemma Comellas and Dr. Anna E. Nesbitt for insightful discussions.

PY - 2013/4

Y1 - 2013/4

N2 - Factor VIII (FVIII, other clotting factors are named similarly) is a glycoprotein that circulates in the plasma bound to von Willebrand factor. During the blood coagulation cascade, activated FVIII (FVIIIa) binds to FIXa and activates FX in the presence of calcium ions and phospholipid membranes. The C1 and C2 domains mediate membrane binding that is essential for activation of the FVIIIa-FIXa complex. Here, 1H, 13C, and 15N backbone chemical shift assignments are reported for the C2 domain of FVIII, including assignments for the residues in solvent-exposed loops. The NMR resonance assignments, along with further structural studies of membrane-bound FVIII, will advance understanding of blood-clotting protein interactions.

AB - Factor VIII (FVIII, other clotting factors are named similarly) is a glycoprotein that circulates in the plasma bound to von Willebrand factor. During the blood coagulation cascade, activated FVIII (FVIIIa) binds to FIXa and activates FX in the presence of calcium ions and phospholipid membranes. The C1 and C2 domains mediate membrane binding that is essential for activation of the FVIIIa-FIXa complex. Here, 1H, 13C, and 15N backbone chemical shift assignments are reported for the C2 domain of FVIII, including assignments for the residues in solvent-exposed loops. The NMR resonance assignments, along with further structural studies of membrane-bound FVIII, will advance understanding of blood-clotting protein interactions.

KW - Blood coagulation

KW - C2 discoidin-like domain

KW - FVIII

KW - Membrane-associated protein

UR - http://www.scopus.com/inward/record.url?scp=84874953150&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84874953150&partnerID=8YFLogxK

U2 - 10.1007/s12104-012-9370-5

DO - 10.1007/s12104-012-9370-5

M3 - Article

C2 - 22392338

AN - SCOPUS:84874953150

SN - 1874-2718

VL - 7

SP - 31

EP - 34

JO - Biomolecular NMR Assignments

JF - Biomolecular NMR Assignments

IS - 1

ER -

Backbone resonance assignments of the C2 domain of coagulation factor VIII

Abstract

Keywords

ASJC Scopus subject areas

Online availability

Library availability

Related links

Fingerprint

Cite this