Back-to-back mechanisms drive actomyosin ring closure during drosophila embryo cleavage

Zenghui Xue, Anna Marie Sokac

Research output: Contribution to journalReview article

Abstract

Contraction of actomyosin rings during cytokinesis is typically attributed to actin filaments sliding toward each other via Myosin-2 motor activity. However, rings constrict in some cells in the absence of Myosin-2 activity. Thus, ring closure uses Myosin-2-dependent and -independent mechanisms. But what the Myosin-2-independent mechanisms are, and to what extent they are sufficient to drive closure, remains unclear. During cleavage in Drosophila melanogaster embryos, actomyosin rings constrict in two sequential and mechanistically distinct phases. We show that these phases differ in constriction speed and are genetically and pharmacologically separable. Further, Myosin-2 activity is required for slow constriction in "phase 1" but is largely dispensable for fast constriction in "phase 2," and F-actin disassembly is only required for fast constriction in phase 2. Switching from phase 1 to phase 2 seemingly relies on the spatial organization of F-actin as controlled by Cofilin, Anillin, and Septin. Our work shows that fly embryos present a singular opportunity to compare separable ring constriction mechanisms, with varying Myosin-2 dependencies, in one cell type and in vivo.

Original languageEnglish (US)
Pages (from-to)335-344
Number of pages10
JournalJournal of Cell Biology
Volume215
Issue number3
DOIs
StatePublished - Jan 1 2016
Externally publishedYes

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Actomyosin
Myosins
Drosophila
Constriction
Embryonic Structures
Actins
Septins
Actin Depolymerizing Factors
Cytokinesis
Drosophila melanogaster
Actin Cytoskeleton
Diptera
Drive
Motor Activity

ASJC Scopus subject areas

  • Cell Biology

Cite this

Back-to-back mechanisms drive actomyosin ring closure during drosophila embryo cleavage. / Xue, Zenghui; Sokac, Anna Marie.

In: Journal of Cell Biology, Vol. 215, No. 3, 01.01.2016, p. 335-344.

Research output: Contribution to journalReview article

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