B-class MADS-box genes in trioecious papaya: Two paleoAP3 paralogs, CpTM6-1 and CpTM6-2, and a PI ortholog CpPI

Christine M. Ackerman, Qingyi Yu, Sangtae Kim, Robert E. Paull, Paul H. Moore, Ray R Ming

Research output: Contribution to journalArticle

Abstract

In the ABC model of flower development, B function organ-identity genes act in the second and third whorls of the flower to control petal and stamen identity. The trioecious papaya has male, female, and hermaphrodite flowers and is an ideal system for testing the B-class gene expression patterns in trioecious plants. We cloned papaya B-class genes, CpTM6-1, CpTM6-2, and CpPI, using MADS box gene specific degenerate primers followed by cDNA library screening and sequencing of positive clones. While phylogenetic analyses show that CpPI is the ortholog of the Arabidopsis gene PI, the CpTM6-1 and CpTM6-2 loci are representatives of the paralogous TM6 lineage that contain paleoAP3 motifs unlike the euAP3 gene observed in Arabidopsis. These two paralogs appeared to have originated from a tandem duplication occurred approximately 13.4 million year ago (mya) (bootstrap range 13.36 ± 2.42). In-situ hybridization and RT-PCR showed that the papaya B-class genes were highly expressed in young flowers across all floral organ primordia. As the flower organs developed, all three B-class genes were highly expressed in petals of all three-sex types and in stamens of hermaphrodite and male flowers. CpTM6-1 expressed at low levels in sepals and carpels, whereas CpTM6-2 expressed at a low level in sepals and at a high level in leaves. Our results showed that B-class gene homologs could function as predicted by the ABC model in trioecous flowers but differential expressions of CpTM6-1, and CpTM6-2, and CpPI suggested the diversification of their functions after the duplication events.

Original languageEnglish (US)
Pages (from-to)741-753
Number of pages13
JournalPlanta
Volume227
Issue number4
DOIs
StatePublished - Mar 1 2008

Fingerprint

Carica
papayas
Genes
flowers
genes
stamens
calyx
Arabidopsis
corolla
male flowers
carpels
Gene Library
cDNA libraries
in situ hybridization
In Situ Hybridization
Clone Cells
reverse transcriptase polymerase chain reaction
clones
screening
flowering

Keywords

  • Carica
  • Molecular phylogeny
  • Nonparametric rate smoothing
  • Tomato MADS-box gene 6 (TM6)
  • paleoAP3 motif

ASJC Scopus subject areas

  • Genetics
  • Plant Science

Cite this

B-class MADS-box genes in trioecious papaya : Two paleoAP3 paralogs, CpTM6-1 and CpTM6-2, and a PI ortholog CpPI. / Ackerman, Christine M.; Yu, Qingyi; Kim, Sangtae; Paull, Robert E.; Moore, Paul H.; Ming, Ray R.

In: Planta, Vol. 227, No. 4, 01.03.2008, p. 741-753.

Research output: Contribution to journalArticle

Ackerman, Christine M. ; Yu, Qingyi ; Kim, Sangtae ; Paull, Robert E. ; Moore, Paul H. ; Ming, Ray R. / B-class MADS-box genes in trioecious papaya : Two paleoAP3 paralogs, CpTM6-1 and CpTM6-2, and a PI ortholog CpPI. In: Planta. 2008 ; Vol. 227, No. 4. pp. 741-753.
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AU - Yu, Qingyi

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AU - Ming, Ray R

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