Auxin regulation involved in gynoecium morphogenesis of papaya flowers

Ping Zhou, Mahpara Fatima, Xinyi Ma, Juan Liu, Ray Ming

Research output: Contribution to journalArticle

Abstract

The morphogenesis of gynoecium is crucial for propagation and productivity of fruit crops. For trioecious papaya (Carica papaya), highly differentiated morphology of gynoecium in flowers of different sex types is controlled by gene networks and influenced by environmental factors, but the regulatory mechanism in gynoecium morphogenesis is unclear. Gynodioecious and dioecious papaya varieties were used for analysis of differentially expressed genes followed by experiments using auxin and an auxin transporter inhibitor. We first compared differential gene expression in functional and rudimentary gynoecium at early stage of their development and detected significant difference in phytohormone modulating and transduction processes, particularly auxin. Enhanced auxin signal transduction in rudimentary gynoecium was observed. To determine the role auxin plays in the papaya gynoecium, auxin transport inhibitor (N-1-Naphthylphthalamic acid, NPA) and synthetic auxin analogs with different concentrations gradient were sprayed to the trunk apex of male and female plants of dioecious papaya. Weakening of auxin transport by 10 mg/L NPA treatment resulted in female fertility restoration in male flowers, while female flowers did not show changes. NPA treatment with higher concentration (30 and 50 mg/L) caused deformed flowers in both male and female plants. We hypothesize that the occurrence of rudimentary gynoecium patterning might associate with auxin homeostasis alteration. Proper auxin concentration and auxin homeostasis might be crucial for functional gynoecium morphogenesis in papaya flowers. These results will lead to further investigation on the auxin homeostasis and gynoecium morphogenesis in papaya.

Original languageEnglish (US)
Article number119
JournalHorticulture Research
Volume6
Issue number1
DOIs
StatePublished - Dec 1 2019

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Carica
gynoecium
Indoleacetic Acids
papayas
Morphogenesis
morphogenesis
auxins
flowers
naphthylphthalamic acid
homeostasis
Homeostasis
Carica papaya
male flowers
female flowers
acid treatment
Genes
Signal transduction
female fertility
Plant Growth Regulators
Gene Regulatory Networks

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Genetics
  • Plant Science
  • Horticulture

Cite this

Auxin regulation involved in gynoecium morphogenesis of papaya flowers. / Zhou, Ping; Fatima, Mahpara; Ma, Xinyi; Liu, Juan; Ming, Ray.

In: Horticulture Research, Vol. 6, No. 1, 119, 01.12.2019.

Research output: Contribution to journalArticle

Zhou, Ping ; Fatima, Mahpara ; Ma, Xinyi ; Liu, Juan ; Ming, Ray. / Auxin regulation involved in gynoecium morphogenesis of papaya flowers. In: Horticulture Research. 2019 ; Vol. 6, No. 1.
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