Autophosphorylation-based calcium (Ca 2+ ) sensitivity priming and Ca 2+ /calmodulin inhibition of Arabidopsis thaliana Ca 2+ -dependent protein kinase 28 (CPK28)

Kyle W. Bender, R. Kevin Blackburn, Jacqueline Monaghan, Paul Derbyshire, Frank L.H. Menke, Cyril Zipfel, Michael B. Goshe, Raymond E. Zielinski, Steven C. Huber

Research output: Contribution to journalArticle

Abstract

Plant calcium (Ca 2+ )-dependent protein kinases (CPKs) represent the primary Ca 2+ -dependent protein kinase activities in plant systems. CPKs are composed of a dual specificity (Ser/Thr and Tyr) kinase domain tethered to a calmodulin-like domain (CLD) via an autoinhibitory junction (J). Although regulation of CPKs by Ca 2+ has been extensively studied, the contribution of autophosphorylation in controlling CPK activity is less well understood. Furthermore, whether calmodulin (CaM) contributes to CPK regulation, as is the case for Ca 2+ /CaM-dependent protein kinases outside the plant lineage, remains an open question. We therefore screened a subset of plant CPKs for CaM binding and found that CPK28 is a high affinity Ca 2+ /CaM-binding protein. Using synthetic peptides and native gel electrophoresis, we coarsely mapped the CaM-binding domain to a site within the CPK28 J domain that overlaps with the known site of intramolecular interaction between the J domain and the CLD. Peptide kinase activity of fully dephosphorylated CPK28 was Ca 2+ -responsive and was inhibited by Ca 2+ /CaM. Using in situ autophosphorylated protein, we expand on the known set of CPK28 autophosphorylation sites, and we demonstrate that, unexpectedly, autophosphorylated CPK28 had enhanced kinase activity at physiological concentrations of Ca 2+ compared with the dephosphorylated protein, suggesting that autophosphorylation functions to prime CPK28 for Ca 2+ activation and might also allow CPK28 to remain active when Ca 2+ levels are low. Furthermore, CPK28 autophosphorylation substantially reduced sensitivity of the kinase to Ca 2+ /CaM inhibition. Overall, our analyses uncover new complexities in the control of CPK28 and provide mechanistic support for Ca 2+ signaling specificity through Ca 2+ sensor priming.

Original languageEnglish (US)
Pages (from-to)3988-4002
Number of pages15
JournalJournal of Biological Chemistry
Volume292
Issue number10
DOIs
StatePublished - Mar 10 2017

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Calmodulin
Arabidopsis
Protein Kinases
Calcium
Phosphotransferases
Calcium-Calmodulin-Dependent Protein Kinases
Calmodulin-Binding Proteins
Peptides
Electrophoresis
Proteins
Gels

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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Autophosphorylation-based calcium (Ca 2+ ) sensitivity priming and Ca 2+ /calmodulin inhibition of Arabidopsis thaliana Ca 2+ -dependent protein kinase 28 (CPK28) . / Bender, Kyle W.; Blackburn, R. Kevin; Monaghan, Jacqueline; Derbyshire, Paul; Menke, Frank L.H.; Zipfel, Cyril; Goshe, Michael B.; Zielinski, Raymond E.; Huber, Steven C.

In: Journal of Biological Chemistry, Vol. 292, No. 10, 10.03.2017, p. 3988-4002.

Research output: Contribution to journalArticle

Bender, KW, Blackburn, RK, Monaghan, J, Derbyshire, P, Menke, FLH, Zipfel, C, Goshe, MB, Zielinski, RE & Huber, SC 2017, ' Autophosphorylation-based calcium (Ca 2+ ) sensitivity priming and Ca 2+ /calmodulin inhibition of Arabidopsis thaliana Ca 2+ -dependent protein kinase 28 (CPK28) ', Journal of Biological Chemistry, vol. 292, no. 10, pp. 3988-4002. https://doi.org/10.1074/jbc.M116.763243
Bender, Kyle W. ; Blackburn, R. Kevin ; Monaghan, Jacqueline ; Derbyshire, Paul ; Menke, Frank L.H. ; Zipfel, Cyril ; Goshe, Michael B. ; Zielinski, Raymond E. ; Huber, Steven C. / Autophosphorylation-based calcium (Ca 2+ ) sensitivity priming and Ca 2+ /calmodulin inhibition of Arabidopsis thaliana Ca 2+ -dependent protein kinase 28 (CPK28) In: Journal of Biological Chemistry. 2017 ; Vol. 292, No. 10. pp. 3988-4002.
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abstract = "Plant calcium (Ca 2+ )-dependent protein kinases (CPKs) represent the primary Ca 2+ -dependent protein kinase activities in plant systems. CPKs are composed of a dual specificity (Ser/Thr and Tyr) kinase domain tethered to a calmodulin-like domain (CLD) via an autoinhibitory junction (J). Although regulation of CPKs by Ca 2+ has been extensively studied, the contribution of autophosphorylation in controlling CPK activity is less well understood. Furthermore, whether calmodulin (CaM) contributes to CPK regulation, as is the case for Ca 2+ /CaM-dependent protein kinases outside the plant lineage, remains an open question. We therefore screened a subset of plant CPKs for CaM binding and found that CPK28 is a high affinity Ca 2+ /CaM-binding protein. Using synthetic peptides and native gel electrophoresis, we coarsely mapped the CaM-binding domain to a site within the CPK28 J domain that overlaps with the known site of intramolecular interaction between the J domain and the CLD. Peptide kinase activity of fully dephosphorylated CPK28 was Ca 2+ -responsive and was inhibited by Ca 2+ /CaM. Using in situ autophosphorylated protein, we expand on the known set of CPK28 autophosphorylation sites, and we demonstrate that, unexpectedly, autophosphorylated CPK28 had enhanced kinase activity at physiological concentrations of Ca 2+ compared with the dephosphorylated protein, suggesting that autophosphorylation functions to prime CPK28 for Ca 2+ activation and might also allow CPK28 to remain active when Ca 2+ levels are low. Furthermore, CPK28 autophosphorylation substantially reduced sensitivity of the kinase to Ca 2+ /CaM inhibition. Overall, our analyses uncover new complexities in the control of CPK28 and provide mechanistic support for Ca 2+ signaling specificity through Ca 2+ sensor priming.",
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AU - Bender, Kyle W.

AU - Blackburn, R. Kevin

AU - Monaghan, Jacqueline

AU - Derbyshire, Paul

AU - Menke, Frank L.H.

AU - Zipfel, Cyril

AU - Goshe, Michael B.

AU - Zielinski, Raymond E.

AU - Huber, Steven C.

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