Automated reading of DNA autoradiogram images using lane profiling methods

K. Palaniappan, Thomas S Huang, H. Lee

Research output: Contribution to conferencePaper

Abstract

Automated DNA sequencing involves computer interpretation of the chemical detection data, which may be in the form of 2-D autoradiogram images, and 1- or 2-D fluorescence data. The analysis of autoradiogram images generated by the multiplex DNA sequencing method is considered. An overall approach to obtaining sequence data from the autoradiogram images is outlined, and specific approaches for segmenting the image into sets of four lanes, obtaining 1-D profiles of the lanes, detecting peaks in the 1-D profiles using a multiscale approach, and aligning profiles across lanes using an interpolation method are discussed. Intermediate results are presented.

Original languageEnglish (US)
Pages360-367
Number of pages8
StatePublished - Dec 1 1989
EventProceedings of the International Workshop on Industrial Applications of Machine Intelligence and Vision - Tokyo, Japan
Duration: Apr 10 1989Apr 12 1989

Other

OtherProceedings of the International Workshop on Industrial Applications of Machine Intelligence and Vision
CityTokyo, Japan
Period4/10/894/12/89

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Palaniappan, K., Huang, T. S., & Lee, H. (1989). Automated reading of DNA autoradiogram images using lane profiling methods. 360-367. Paper presented at Proceedings of the International Workshop on Industrial Applications of Machine Intelligence and Vision, Tokyo, Japan, .