Autohydrolysis of Miscanthus x giganteus for the production of xylooligosaccharides (XOS): Kinetics, characterization and recovery

Ming Hsu Chen, Michael J. Bowman, Bruce S. Dien, Kent D. Rausch, M. E. Tumbleson, Vijay Singh

Research output: Contribution to journalArticle

Abstract

The optima conditions of production and purification of xylooligosaccharides (XOS) from Miscanthus x giganteus (MxG) were investigated. Using autohydrolysis, XOS were produced at 160, 180 and 200. °C at 60, 20 and 5. min, respectively. XOS yield up to 13.5% (w/w) of initial biomass and 69.2% (w/w) of xylan were achieved. Results from HPAEC-PAD analysis revealed that X1-X9 sugar oligomers were produced. Higher temperature and longer reaction time resulted in lower product molecular weight. The three optimum conditions had similar degrees of polymerization XOS. Using 10% activated carbon (w/v) with ethanol/water elution recovered 47.9% (w/w) of XOS from pretreated liquid phase. The XOS could be fractionated by degree of polymerization according to ethanol concentration in the ethanol/water elution. Most of the XOS were washed out in 30% and 50% ethanol/water (v/v) fractions. Recoveries of 91.8% xylobiose, 86.9% xylotriose, 66.3% xylotetrose, 56.2% xylopentose and 48.9% xylohexaose were observed in XOS.

Original languageEnglish (US)
Pages (from-to)359=365
JournalBioresource Technology
Volume155
DOIs
StatePublished - Mar 2014

Keywords

  • Autohydrolysis
  • Degree of polymerization (DP)
  • Miscanthus x giganteus
  • Pretreatment
  • Xylooligossaccharides (XOS)

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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