Attenuation of Cell Mechanosensitivity in Colon Cancer Cells during In Vitro Metastasis

Xin Tang, Qi Wen, Theresa B. Kuhlenschmidt, Mark S. Kuhlenschmidt, Paul A. Janmey, Taher A. Saif

Research output: Contribution to journalArticle

Abstract

Human colon carcinoma (HCT-8) cells show a stable transition from low to high metastatic state when cultured on appropriately soft substrates (21 kPa). Initially epithelial (E) in nature, the HCT-8 cells become rounded (R) after seven days of culture on soft substrate. R cells show a number of metastatic hallmarks [1]. Here, we use gradient stiffness substrates, a bio-MEMS force sensor, and Coulter counter assays to study mechanosensitivity and adhesion of E and R cells. We find that HCT-8 cells lose mechanosensitivity as they undergo E-to-R transition. HCT-8 R cells' stiffness, spread area, proliferation and migration become insensitive to substrate stiffness in contrast to their epithelial counterpart. They are softer, proliferative and migratory on all substrates. R cells show negligible cell-cell homotypic adhesion, as well as non-specific cell-substrate adhesion. Consequently they show the same spread area on all substrates in contrast to E cells. Taken together, these results indicate that R cells acquire autonomy and anchorage independence, and are thus potentially more invasive than E cells. To the best of our knowledge, this is the first report of quantitative data relating changes in cancer cell adhesion and stiffness during the expression of an in vitro metastasis-like phenotype.

Original languageEnglish (US)
Article numbere50443
JournalPloS one
Volume7
Issue number11
DOIs
StatePublished - Nov 30 2012

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colorectal neoplasms
metastasis
Colonic Neoplasms
Cells
Neoplasm Metastasis
Substrates
Stiffness
cells
Cell adhesion
Cell Adhesion
Adhesion
cell adhesion
adhesion
neoplasm cells
In Vitro Techniques
Micro-Electrical-Mechanical Systems
MEMS
Assays
Sensors
carcinoma

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Attenuation of Cell Mechanosensitivity in Colon Cancer Cells during In Vitro Metastasis. / Tang, Xin; Wen, Qi; Kuhlenschmidt, Theresa B.; Kuhlenschmidt, Mark S.; Janmey, Paul A.; Saif, Taher A.

In: PloS one, Vol. 7, No. 11, e50443, 30.11.2012.

Research output: Contribution to journalArticle

Tang, Xin ; Wen, Qi ; Kuhlenschmidt, Theresa B. ; Kuhlenschmidt, Mark S. ; Janmey, Paul A. ; Saif, Taher A. / Attenuation of Cell Mechanosensitivity in Colon Cancer Cells during In Vitro Metastasis. In: PloS one. 2012 ; Vol. 7, No. 11.
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