Abstract
Histopathology forms the gold standard for the diagnosis of breast cancer. Fourier Transform Infrared (FT-IR) spectroscopic imaging has been proposed to be a potentially powerful adjunct to current histopathological techniques. Most studies using FT-IR imaging for breast tissue analysis have been in the transmission or transmission-reflection mode, in which the wavelength and optics limit the data to a relatively coarse spatial resolution (typically, coarser than 5 μm × 5 μm per pixel). This resolution is insufficient to examine many histologic structures. Attenuated total reflectance (ATR) FT-IR imaging incorporating a Germanium optic can allow for a four-fold increase in spatial resolution due to the material's high refractive index in the mid-IR. Here, we employ ATR FT-IR imaging toward examining cellular and tissue structures that constitute an important component of breast cancer diagnosis. In particular, we resolve and chemically characterize endothelial cells, myoepithelial cells and terminal ductal lobular units. Further extending the ability of IR imaging to examine sub-cellular structures, we report the extraction of intact chromosomes from breast cancer cells and their spatially localized analysis as a novel approach to understand changes associated with the molecular structure of DNA in breast cancer.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 23-28 |
| Number of pages | 6 |
| Journal | Vibrational Spectroscopy |
| Volume | 60 |
| DOIs | |
| State | Published - May 1 2012 |
Fingerprint
Keywords
- Attenuated total reflection
- Breast cancer
- Cells
- DNA
- Fourier-transform infrared spectroscopy
- Tissue imaging
ASJC Scopus subject areas
- Spectroscopy
Cite this
Attenuated total reflectance Fourier-transform infrared spectroscopic imaging for breast histopathology. / Walsh, Michael J.; Holton, Sarah E.; Kajdacsy-Balla, Andre; Bhargava, Rohit.
In: Vibrational Spectroscopy, Vol. 60, 01.05.2012, p. 23-28.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Attenuated total reflectance Fourier-transform infrared spectroscopic imaging for breast histopathology
AU - Walsh, Michael J.
AU - Holton, Sarah E.
AU - Kajdacsy-Balla, Andre
AU - Bhargava, Rohit
PY - 2012/5/1
Y1 - 2012/5/1
N2 - Histopathology forms the gold standard for the diagnosis of breast cancer. Fourier Transform Infrared (FT-IR) spectroscopic imaging has been proposed to be a potentially powerful adjunct to current histopathological techniques. Most studies using FT-IR imaging for breast tissue analysis have been in the transmission or transmission-reflection mode, in which the wavelength and optics limit the data to a relatively coarse spatial resolution (typically, coarser than 5 μm × 5 μm per pixel). This resolution is insufficient to examine many histologic structures. Attenuated total reflectance (ATR) FT-IR imaging incorporating a Germanium optic can allow for a four-fold increase in spatial resolution due to the material's high refractive index in the mid-IR. Here, we employ ATR FT-IR imaging toward examining cellular and tissue structures that constitute an important component of breast cancer diagnosis. In particular, we resolve and chemically characterize endothelial cells, myoepithelial cells and terminal ductal lobular units. Further extending the ability of IR imaging to examine sub-cellular structures, we report the extraction of intact chromosomes from breast cancer cells and their spatially localized analysis as a novel approach to understand changes associated with the molecular structure of DNA in breast cancer.
AB - Histopathology forms the gold standard for the diagnosis of breast cancer. Fourier Transform Infrared (FT-IR) spectroscopic imaging has been proposed to be a potentially powerful adjunct to current histopathological techniques. Most studies using FT-IR imaging for breast tissue analysis have been in the transmission or transmission-reflection mode, in which the wavelength and optics limit the data to a relatively coarse spatial resolution (typically, coarser than 5 μm × 5 μm per pixel). This resolution is insufficient to examine many histologic structures. Attenuated total reflectance (ATR) FT-IR imaging incorporating a Germanium optic can allow for a four-fold increase in spatial resolution due to the material's high refractive index in the mid-IR. Here, we employ ATR FT-IR imaging toward examining cellular and tissue structures that constitute an important component of breast cancer diagnosis. In particular, we resolve and chemically characterize endothelial cells, myoepithelial cells and terminal ductal lobular units. Further extending the ability of IR imaging to examine sub-cellular structures, we report the extraction of intact chromosomes from breast cancer cells and their spatially localized analysis as a novel approach to understand changes associated with the molecular structure of DNA in breast cancer.
KW - Attenuated total reflection
KW - Breast cancer
KW - Cells
KW - DNA
KW - Fourier-transform infrared spectroscopy
KW - Tissue imaging
UR - http://www.scopus.com/inward/record.url?scp=84861094876&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84861094876&partnerID=8YFLogxK
U2 - 10.1016/j.vibspec.2012.01.010
DO - 10.1016/j.vibspec.2012.01.010
M3 - Article
AN - SCOPUS:84861094876
VL - 60
SP - 23
EP - 28
JO - Vibrational Spectroscopy
JF - Vibrational Spectroscopy
SN - 0924-2031
ER -