Attenuated total reflectance Fourier-transform infrared spectroscopic imaging for breast histopathology

Michael J. Walsh, Sarah E. Holton, Andre Kajdacsy-Balla, Rohit Bhargava

Research output: Contribution to journalArticle

Abstract

Histopathology forms the gold standard for the diagnosis of breast cancer. Fourier Transform Infrared (FT-IR) spectroscopic imaging has been proposed to be a potentially powerful adjunct to current histopathological techniques. Most studies using FT-IR imaging for breast tissue analysis have been in the transmission or transmission-reflection mode, in which the wavelength and optics limit the data to a relatively coarse spatial resolution (typically, coarser than 5 μm × 5 μm per pixel). This resolution is insufficient to examine many histologic structures. Attenuated total reflectance (ATR) FT-IR imaging incorporating a Germanium optic can allow for a four-fold increase in spatial resolution due to the material's high refractive index in the mid-IR. Here, we employ ATR FT-IR imaging toward examining cellular and tissue structures that constitute an important component of breast cancer diagnosis. In particular, we resolve and chemically characterize endothelial cells, myoepithelial cells and terminal ductal lobular units. Further extending the ability of IR imaging to examine sub-cellular structures, we report the extraction of intact chromosomes from breast cancer cells and their spatially localized analysis as a novel approach to understand changes associated with the molecular structure of DNA in breast cancer.

Original languageEnglish (US)
Pages (from-to)23-28
Number of pages6
JournalVibrational Spectroscopy
Volume60
DOIs
StatePublished - May 1 2012

Fingerprint

Infrared imaging
Fourier transforms
Infrared radiation
Imaging techniques
Optics
Tissue
Germanium
Wave transmission
Endothelial cells
Chromosomes
Molecular structure
Refractive index
Pixels
Cells
Wavelength
DNA

Keywords

  • Attenuated total reflection
  • Breast cancer
  • Cells
  • DNA
  • Fourier-transform infrared spectroscopy
  • Tissue imaging

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Attenuated total reflectance Fourier-transform infrared spectroscopic imaging for breast histopathology. / Walsh, Michael J.; Holton, Sarah E.; Kajdacsy-Balla, Andre; Bhargava, Rohit.

In: Vibrational Spectroscopy, Vol. 60, 01.05.2012, p. 23-28.

Research output: Contribution to journalArticle

Walsh, Michael J. ; Holton, Sarah E. ; Kajdacsy-Balla, Andre ; Bhargava, Rohit. / Attenuated total reflectance Fourier-transform infrared spectroscopic imaging for breast histopathology. In: Vibrational Spectroscopy. 2012 ; Vol. 60. pp. 23-28.
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