ATP stimulation of the sn-glycerol-3-phosphate acyltransferase of Escherichia coli

Charles O. Rock, Susan E. Goelz, John E. Cronan

Research output: Contribution to journalArticlepeer-review


ATP was found to stimulate the rate of the inner membrane sn-glycerol-3-phosphate acyltransferase of Escherichia coli. Stimulation required the presence of Mg2+ and was demonstrated with either coenzyme A or acyl carrier protein thioesters as the acyl donor. The ATP stimulation was consistently observed in freshly prepared membranes and those stored at 4 °C, but after freeze/thaw treatment, the acyltransferase no longer responded to ATP. ATP increased the maximal velocity of the reaction but did not affect the Michaelis constants of the substrates. ATP did not drastically alter the proportions or types of products formed in the reaction. The ATP effect may be a mechanism functioning to enhance the rate of the acyltransferase reaction in response to an increased supply of metabolic energy.

Original languageEnglish (US)
Pages (from-to)113-118
Number of pages6
JournalArchives of Biochemistry and Biophysics
Issue number1
StatePublished - Oct 1 1981

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology


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