Assignment of the agent of Tyzzer's disease to Clostridium piliforme comb. nov. on the basis of 16S rRNA sequence analysis

A. J. Duncan, R. J. Carman, Gary J Olsen, K. H. Wilson

Research output: Contribution to journalArticle

Abstract

The small-subunit rRNA (16S rRNA) sequence of Tyzzer's bacillus (also known as 'Bacillus piliformis') was elucidated by using the polymerase chain reaction followed by reverse transcriptase sequencing. By using maximum- likelihood analysis, a phylogenetic tree was constructed from this and other 16S rRNA sequences available from the first release of the Ribosomal Database Project (G. J. Olsen, R. Overbeek, N. Larsen, T. L. Marsh, M. J. McCaughey, M. A. Maciukenas, W.-M. Kuan, T. J. Macke, Y. Xing, and C. R. Woese, Nucleic Acids Res. 20:2199-2200, 1992). Tyzzer's bacillus grouped with a specific set of anaerobic bacteria, most of which are Clostridium spp. The closest identified relatives are Clostridium coccoides, Clostridium oroticum, Clostridium clostridiiforme, Clostridium symbiosum, and Streptococcus hansenii. Clostridium amino-valericum and 'Acetitomaculum ruminis' are also solidly allied with this ensemble. We propose that Tyzzer's bacillus be reclassified as Clostridium piliforme on the basis of its 16S rRNA sequence.

Original languageEnglish (US)
Pages (from-to)314-318
Number of pages5
JournalInternational Journal of Systematic Bacteriology
Volume43
Issue number2
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

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Clostridium piliforme
maximum likelihood analysis
Comb and Wattles
Clostridium
nucleic acid
polymerase chain reaction
Sequence Analysis
marsh
sequence analysis
ribosomal RNA
phylogenetics
Bacillus
bacterium
Ruminococcus hansenii
Acetitomaculum ruminis
Clostridium clostridioforme
Clostridium coccoides
Clostridium symbiosum
probability analysis
RNA-directed DNA polymerase

ASJC Scopus subject areas

  • Microbiology
  • Immunology

Cite this

Assignment of the agent of Tyzzer's disease to Clostridium piliforme comb. nov. on the basis of 16S rRNA sequence analysis. / Duncan, A. J.; Carman, R. J.; Olsen, Gary J; Wilson, K. H.

In: International Journal of Systematic Bacteriology, Vol. 43, No. 2, 01.01.1993, p. 314-318.

Research output: Contribution to journalArticle

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